Using Buffers Gino A. Romeo‚ Jr.‚ Ph.D. Version 42-0134-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
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Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic acid‚ Gylcine
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Conclusion Both Kool Aid and Gatorade contain buffers. For the buffer in Kool Aid‚ pKa1 ≈ 3.70‚ pKa2 ≈ 4.90‚ pKa3 ≈ 6.50 and for the buffer in Gatorade‚ pKa1 ≈ 4.50‚ pKa2 ≈ 5.40‚ pKa3 ≈ 6.70. Discussion of Theory A buffer capacity is the maximum amount of hyrdrogen or hydroxide ion that can be added to a buffered solution before a significant change in the pH occurs. Increasing the concentration of the buffer can increase the buffer capacity of a solution. At the half equivalence point‚ the moles
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t Design and preparation of buffers effective at different pHs Abstract These experiments aimed to determine the optimum pH ranges various buffers are effective and provide opportunity for the use of the Henderson-Hasselbalch equation to prepare a buffer of a specific pH. Three different buffer systems were initially investigated; volumes of weak acid and weak bases of specified concentration were prepared and titrated against strong acid or strong base solutions with pH readings taken at frequent
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Materials: Brightly colored test tube rack‚ kim wipes‚ beaker for trash 1.5 ml microcentrifuge tubes Test plate Micropipetters and tips DI water Buffer solution … 4.5 to 8.8 I2Kl (grams iodine) Starch solution Enzyme (amylase) 80 degree Celsius water (HOT) Floating test rack Procedure: While controlling the amount of starch and the amount of buffer we use with a pH of 5.8‚ we want to investigate how changes in enzyme concentration affect reaction rates. First we put 500 ml of amylase from
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Title: Mitosis and Meiosis Abstract: There are two types of nuclear division‚ mitosis and meiosis. Mitosis is usually used for the growth and replacement of somotaic cells‚ while meiosis produces the gametes or spores used in an organism’s reproduction. Hypothesis: Mitosis occurs in whitefish blastula and onion root tip‚ and it is easily observable. Meiosis and crossing over occurs in the production of gametes and spores. Materials: This lab required prepared slides of whitefish blastula
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Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate student’s writing of lab reports by providing this information in an editable file which can be sent to an instructor. Exercise
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Lab Manual Anatomy and Physiology LabPaq: AP-1 14 Small-Scale Experiments for Independent Study Published by Hands-On Labs‚ Inc. Anatomy and Physiology: Independent Laboratory Exercises for the First Semester Designed to accompany Anatomy & Physiology LabPaq AP-1 062211 LabPaq® is a registered trademark of Hands-On Labs‚ Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs‚ Inc. which holds and reserves all copyrights on the intellectual properties associated
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---------------------------------- Acidspunk installation instruction ---------------------------------- Acidspunk requires an MMX processor and DirectX Place the .dll in winamp’s plugin directory. Start winamp and press "ctrl-k" Choose acidspunk and press "configure" (or "alt-k") Set the screen resolution Start the plugin "ctrl-shift-k" Check the framerate by pressing F2 while the plugin is running. The plugin should run between 20-30 frames per second to look good. If not adjust
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[pic] Drops |Water(HcL) |Water(NaOH) |Liver(HcL) |Liver(NaOH) |Egg White(HcL) |Egg White(NaOH0) |Potato(HCl) |Potato(NaOH) |Buffer(HCl) |Buffer(NaOH) | |0 |7 |4 |7.4 |5 |8.2 |7 |6.9 |4 |10.7 |10 | |5 |4.5 |7 |6.9 |6 |7.5 |8 |6.2 |5 |10.5 |10 | |10 |2.7 |9 |6.3 |6 |7 |9 |5.7 |5 |10.4 |11 | |15 |2.6 |12 |5.8 |6 |6.4 |9 |5.3 |6 |10.3 |12 | |20 |2.5 |12 |5.4 |6 |4.5 |10 |4.9 |7 |10.2 |12 | |25 |2.4 |13 |5.1 |6 |3.5 |10 |4.6 |8 |10.1 |13 | |30 |2.3 |13 |4.8 |6 |3.3 |11 |4.2 |8 |10 |13 | | 1.
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