ECS-165A WQ’11 110 6. Storage and File Structures Goals Understand the basic concepts underlying different storage media‚ buffer management‚ files structures‚ and organization of records in files. Contents • • • • • Overview of Physical Storage Media Magnetic Disks‚ Tertiary Storage Buffer Management Storage Access File Organization Dept. of Computer Science UC Davis 6. Storage and File Organization ECS-165A WQ’11 111 Classification of Physical Storage Media • Main criteria: Speed with
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This prevents the samples from overflowing in the gel. Once this was completed the gel was moved so that the wells were on the negative electrode side. Then 300mL of Triacetate EDTA Buffer with a pH of 8.0 was then added to the electrophoresis apparatus. The wires for the electrophoresis apparatus were then connected to the voltage base; one wire on the negative and the other on the positive. Then cover the gel with the lid and turning
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Properties of Gases CHE101 Experiment date: 06/20 Write-up date: 06/21-06/22 Purpose: The purpose of this experiment is to examine different properties of gases in order to be able to identify them in a laboratory setting. I will be using Hydrochloric acid‚ mossy zinc‚ Bromothymol blue‚ limewater‚ Manganese‚ and Alka Seltzer‚ to test different properties of gases formed by chemical reactions. In order to extract the gas I will use several household products such as vinegar‚ baking soda‚ water
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to the acidity. MATERIALS AND METHODS I followed the methods in the Cells and Molecules Lab Manual on pages 82-87. RESULTS Table 1: Reaction Mixtures for Standardization of Peroxidase; Absorbance Data Tube pH 5 buffer Peroxidase solution Guaiacol solution Turnip extract total volume Absorbance 20” 40” 60” 80” 100”
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Preformulation activities range from supporting identification of new active agents to characterizing physical properties necessary for the design of dosage forms. Critical information provided during the preformulation can enhance the rapid and successful introduction of new therapeutic entities for humans. Hence‚ preformulation studies are essential to characterize drugs for proper designing of drug delivery systems. The preformulation studies‚ which were performed in this project‚ include – identification
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LAB 6. ACIDS AND BASES: PH AND BUFFERS PURPOSE: To determine the pH of common acids and bases using a pH meter‚ pH paper‚ and red cabbage indicator. To test the effect of adding an acid or base to a buffer solution. SAFETY CONCERNS: Always wear safety goggles. Wash with soap and water if skin contacts acids or bases. ACIDS: An Acid is a substance that when dissolved in water will produce hydrogen ions‚ H+‚ in the solution. An acid that does not contain carbon is called an inorganic
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Amylase Amylase Amylase Amylase 5 Deionized Water Deionized Water pH 2.0 Buffer Amylase 6 7 8 Reagent 2 Starch Starch Starch Deionized Water Starch Reagent 3 pH 7.0 Buffer pH 7.0 Buffer pH 7.0 Buffer pH 7.0 Buffer Treatment Boiled Frozen None None Time 60 60 60 60 Temp. 37 37 37 37 IKI + - Benedict’s ++ ++ - pH 7.0 Buffer None 60 37 + - Maltose pH 7.0 Buffer None 60 37 - ++ Starch Starch Amylase pH 9.0 Buffer None None 60 60 37 37 + + + + 04/08/15 page 2 Post-lab Quiz
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3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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Peel samples‚ coming from Ananas comosus‚ will undergo a series of physical and chemical analyses to determine the glucose content‚ which will be utilized to yield bioethanol. The SSF process will be manipulated in terms of fermentation time and buffer level used to determine the best variables for biofuel production. Introduction A. Background of the Study The concept of producing bioethanol from pineapple (Anae ananas comosus)
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Nick Sarris‚ April 3‚ 2013‚ D-Bell Biology Virtual Electrophoresis Lab – Genetic Science Learning Center Use the link to complete the following lab. Submit through edline when you are finished http://learn.genetics.utah.edu/units/biotech/gel/ Title‚ name‚ date and bell (8 pts) Place your answer below the question and skip between questions (2 pts) Each question is worth 3 points 1. Why can’t DNA be sorted physically‚ using a microscope?- They are so tiny that they are unable to be
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