substitute ways to meet demand.( OM Triangle) Inventory build up without variability Scenario 1: Demand rate < Capacity‚ and no buffer inventory Throughput rate = Demand rate Scenario 2: Demand rate > Capacity Buffer inventory builds up at rate: Demand rate – Capacity Throughput rate = Capacity rate Scenario 3: Demand rate < Capacity‚ buffer inventory exists Buffer inventory depletes at rate: Capacity – Demand rate Throughput rate = Capacity rate Throughput rate is what actually happened
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are summed‚ the variance of the sum equals the sum of the variances of individual distributions. Answer: TRUE Diff: 2 Section: 11.4 The Critical Chain Solution to Project Scheduling Skill: Analytical AACSB Tag: Analytic Skills 7) A feeder buffer is a buffer that is created for a non-critical path activity. Answer: TRUE Diff: 1 Section: 11.4 The Critical Chain Solution to Project Scheduling Skill: Definition AACSB Tag: Reflective 8) An
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on the same graph. (2 marks) Q3. Define the term Tm. Describe the melting curve of DNA in TE buffer and indicate its Tm value. (2 marks) Tm is the temperature at which half of the DNA strands are in the double-helical state and of them are in the "random-coil" state. It can be determined from the following equation‚ Aaverage = (Amax + Amin) /2 The melting curve of DNA in TE buffer is in sigmoidal shape and can be divided into three parts. The first part of the curve (20oC-60oC)
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contained a population of Gram negative bacteria. Each sample was placed in an HEPES buffer at a pH of 7.2 and concentration of 1.0 mM. This created a hypotonic solution. The first and second group contained three samples each. The first sample was a control and contained only the buffer and the Micrococcus. The second sample contained the buffer‚ the Micrococcus‚ and the lysozyme. The third sample contained the buffer‚ the Micrococcus‚ the lysozyme‚ and KCl used to create an isotonic
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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chemical compound‚ write the IUPAC name (no common names). 2. Given the IUPAC name of an ionic or molecular compound‚ write the chemical formula associated with it. Materials: Student Provides: The exercises below. From LabPaq: Nothing required. Discussion: At this point in the course you have used the periodic table as your reference for where the elements are located. The Periodic Table will be the most important tool to have on hand for this exercise and for every
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graph shows RFU’s (left) and total protein in ug (right) for each wash and elution. Once the rGFP had been purified in the Ni2+-agarose column‚ a breaking buffer (10mM Tris‚ pH 8.0; 150mM NaCl) was used to create the washes by pipetting the buffer in 0.5ml increments and collecting each in separate tubes labeling them W1-W6. Then‚ an elution buffer (10mM Tris‚ pH 8.0; 150mM NaCl; 300mM imidazole) was collected in increments of 0.5ml and labeled E1-E6. We could measure the amount of RFU’s each sample
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Our results indicated that at three different pH levels‚ the absorbance level of our samples was not the same for each one. This proved my original hypothesis to be incorrect‚ as I was surprised to find that the last pH buffer had no effect on the absorbance. The first two pH buffers supported my hypothesis‚ however. The levels of our samples kept decreasing over time. As with every experiment‚ it should be repeated several times to make sure your results are accurate. Introduction Most chemical
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Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
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leaders of the empire devised three ways to protect the empire from the barbarians. B. The leaders of the empire devised three ways to deal with the Huns and Mongols. III. The end of the empires A. Toward the end of the empire’s rule‚ some of the buffer states revolted brought on the empire’s breakdown. B. The barbarians broke through to the empire and set up dynasties imitating the Chinese way of life. Roman and Chinese Empires We know about some great
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