Practical Exam Survival Guide Spectrophotometry * Know to create a calibration curve: What are the labels for the axes? (Absorbance on y-axis‚ concentration on the x-axis.) * Know how to use the data from a calibration curve to find (1) slope and (2) equation of the line. * How are absorbance and transmittance related? We discussed several equations: A = log (100/%T) A = (I0/IT) T = (IT/I0) x 100% A = 2 – log (%T) * What is Beer’s law and what does it describe? Rates
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sulfate was slowly added for a 65% cut precipitate and was stirred slowly for an additional 15 minutes. The 65% cut was centrifuged for 15 minutes at 15‚000 xg. The supernatant was discarded. The pellet was resuspended in cold 4 mL of pH 7.5 phosphate buffer and transferred to a pre-chilled test tube for further purification. 200 uL was frozen down for later
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Reagent 1 Reagent 2 Reagent 3 Boiled Time Temp. 1 2 3 4 5 6 Pepsin Pepsin Pepsin DeIonized Water Pepsin Pepsin BAPNA BAPNA DeIonized Water BAPNA BAPNA BAPNA pH 2.0 Buffer pH 2.0 Buffer pH 2.0 Buffer pH 2.0 Buffer pH 7.0 Buffer pH 9.0 Buffer + - 60 60 60 60 60 60 37 37 37 37 37 37 09/22/13 page 2 Optical Density 0.00 0.40 0.00 0.00 0.03 0.00 Post-lab Quiz Results You scored 75% by answering 3 out of 4 questions
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The effect temperature has on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts‚ which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical reactions that occur within the human body. The reason in which enzymes are sensitive to heat‚ pH and heavy metal ions is because they are made up of proteins. The food we eat is turned into energy by enzymes
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is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions were compared‚ a purchased control ConA solution in buffer as the positive control‚ and a purified solution of ConA in buffer previously purified in lab. Each solution was at a 2mg/ml concentration of ConA in ConA buffer‚ which is necessary for ConA’s biological activity. Two variables were added‚ Galactose and Mannose‚ to the ConA solution to compare the effects each had on the hemagglutination
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negative net charge will move toward the positive electrode. An inert substance such as paper or gel is used as a supporting medium for the conducting liquid in most electrophoretic methods. In this experiment of separating amino acids‚ a phosphate buffer (pH 6) will be used as the conducting liquid and cellulose as the supporting medium. Purpose: The purpose of this experiment is to determine the effect of an electrical field on charged particles and to use this information to identify the amino
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Biology Controlled Assessment. Kenishia Pascal 10x3. Investigating How Different Concentration Effects The Rate Of Reaction. Strategy A Possible Factors * Source of catalase * Concentration * Surface Area of enzyme * Concentration of enzyme * pH * Temperature Chosen Factor We chose to investigate the concentration of enzyme as we had previously investigated the optimum temperature for catalase in the preliminary investigation. Concentration of enzyme
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may use a scientific calculator for this test. When you have finished‚ submit this assignment to your teacher by the due date for full credit. (5 points) 1. Explain what is in a buffer. Discuss the function of a buffer. How will pH change when small amounts of acids or bases are added to the buffer solution? Answer: A buffer is an aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. Its pH changes very little when a small amount of strong
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The Endocrine System Exercise 1: The Pituitary Gland obseRvations A. Please describe in detail the structures and features you observed on the pituitary gland slide. The Pituitary Gland Slides The Anterior Pituitary Gland was darker than the Posterior Pituitary Gland. In the Anterior Pituitary Gland Slide you can see Chromophobes and Acidophiles. Not super easy to distinguish which is which though. The Posterior Pituitary Gland slide showed Pitulcytes (Neuroglia) as light purple
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shown in fig. • The 8-bit data bus buffer is controlled by the read/write control logic. The read/write control logic manages all of the internal and external transfers of both data and control words. • RD‚ WR‚ A1‚ A0 and RESET are the inputs provided by the microprocessor to the READ/ WRITE control logic of 8255. The 8-bit‚ 3-state bidirectional buffer is used to interface the 8255 internal data bus with the external system data bus. PIO 8255 • This buffer receives or transmits data upon the
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