Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed with salt and Pantene Pro-V Shampoo. The buffer degrades the protein enveloping the DNA found in onion and cheek cells Cheek cells are obtained from gargled gatorade. Extracts are then filtered and subjected to addition of chilled ethanol
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of buffer‚ 2.0mL of H2O2‚ and 1.0 mL of guaiacol. Tube 1 will be to calibrate the spectrometer. Tube 2-3 will be filled with Dil 0.25‚ tube 2‚ 4‚ 6 & 8 will contains 2.0 of 10mM H2O2 and 1.0 of 25mM of guaiacol. Tube 3 will contain 4.75 of 0.1 Buffer in mL and 0.25 of enzyme extract in mL. Tube 4-5 will be filed with Dil 0.5‚ but tube 5 will contain 4.5 of 1.0 Buffer in mL and 0.5 of enzyme extract in mL. Tube 6-7 will be filled with Dil 1.0‚ for instant tube 7 will contain 4.0 of 0.1 Buffer in mL
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IMMUNIZATION PROGRAM IN INDIA – POLIO VACCINATION Trimester 7 Supply Chain Management – Group Project Prepare by: Group 9 Abhishek Sharma (WMP4003) Akshat Jain (WMP4007) Deepak Gupta (WMP4024) Srinivas D. (WMP4055) Contents What is Universal Immunization Programme ............................................................... 2 Supply Chain Management – Scoping .......................................................................... 3 Demand Estimation ..........................
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reserves were created in the country on a strategy of "core- buffer". The main sectors were exempted from any kind of human activity and buffer zones have been subjected to "land use to conserve." Management plans were developed for each tiger reserve‚ based on the principles set out below: 1. Elimination of all forms of human exploitation and biotic disturbance from the central and rationalization of activities in the buffer zone. 2. Restricting the habitat management only to repair
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Bio 104-03 Lab #3- Acids Bases‚ and Buffers 1. An acid is a substance that releases hydrogen ions as they desolve in water and ranks lower than seven on the pH scale. An example of an acidic substance is lemon juice. A base is a substance that accepts hydrogen ions and ranks higher than seven on the pH scale. An example of a base substance is hand soap. A neutral substance ranks a seven on the pH scale and has equal concentrations of H+ and OH-. An example of this is pure water. Electrolytes
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Applying Bovine serum albumin (BSA) (up to 3%) in the rung buffer could be useful. It is can be loaded into the running buffer and run with the target analytes while a interaction analysis of BSA binding on SPR sensor chip is needed in order to eliminate the signal generated from BSA binding at the final calculation. BSA is the most preferred
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of the cheek was then chewed for approximately 30 seconds. The 3 mL of water was used to rinse the mouth for 30 seconds‚ after which the water and the cheek cells were expelled back into the 15 mL tube. Using a plastic transfer pipet‚ 2 mL of lysis buffer was added to the tube containing the water with the cheek cells. The tube was then capped and gently inverted 5 times to lyse the cells. Five drops of protease and salt solution were added to the sample. The tube was capped and inverted 5 times.
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with the CPU?) Description A tiny computer chip‚ called the keyboard controller or keyboard bios‚ that a key has been pressed. The keyboard controller places a code into a part of its memory‚ called the keyboard buffer‚ indicating which key was pressed. This code is called the key’s scan code. The keyboard controller then signals the computer’s system software that something has happened at the keyboard. It does not specify what has occurred‚ just that something
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will makes the plasmid which contains desired gene more easily to be isolated from self-ligated plasmid. The bacteria plasmid DNA that being extracted is from E. coli by applying miniprep method. Solution I‚ Solution II‚ Solution III‚ ethanol and TE buffer are miniprep reagent. The inoculums of E. coli was undergoes the first centrifugation under 12‚000rpm to separate out the
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1. PURPOSE: The purpose of this SOP is to address the clearing‚ disassembling‚ cleaning‚ and inspecting for serviceability‚ lubricating‚ and reassembling of the M4A1 carbine. 2. APPLICABILITY: This SOP applies to all personnel assigned to 3rd Platoon. 3. REFERENCES: a. Lubricate Order (LO) b. STP 21–1–SMCT c. TM 9–1005–319–10 d. DA Form 2404 4. RESPONSIBILITIES: Every soldier assigned to 3rd Platoon‚ HHD‚ 212th CSH‚ will be responsible for properly clearing‚ disassembling
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