Introduction Plasmids are circular‚ double stranded extrachromosomal DNA molecules that are found in bacteria which can self-replicate. They are naturally occurring DNA molecules advantageous to the host bacterium by carrying genes which specify metabolic capacities. (Garrett et al.‚ 2010) Besides‚ plasmids exist in a wide variety of sizes from a few thousands to hundreds of thousands of base pairs. Many plasmids have been engineered to serve as plasmids cloning vectors to carry genes. (Synder et
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Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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of EDTA and then to use this sample to determine the hardness of a sample of water. This was done using a titrimetric method. This was standardized using calcium chloride. The calcium chloride was weighed‚ dissolved and diluted to a known volume. A buffer and indicator were added to the solution which was then titrated with the EDTA. The water sample was then used as the titrating solution and the procedure repeated. The hardness of water was determined and was found to be 9.25 x 104 ppm. Introduction:
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REFUSING REQUEST Writing plan for a request or claim BUFFER – identifies previous correspondence incidentally or in subject line - begins with a neutral statement on which both reader and writer can agree TRANSITION- plants key idea or word - leads naturally to explanation EXPLANATION- presents valid reasons for refusal - avoids red-flag words that forecasts bad news - includes resale or sales promotion material if appropriate BAD NEWS- softens the blow
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negative on blue. A buffer is added to acidic or base substances to get them to a certain pH state and prevent change from happening. This is important in agriculture‚ food processing‚ and medicine. Our body uses buffers to make sure our blood has a pH of 7. We also use buffers in reactions involving enzymes. Conclusion- After finishing this lab the data and results contained the pH of all the substances we tested and their effect on litmus paper. We also tested buffers and how
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An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase‚ during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic
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6 – 150 mL beakers were set up and a stir bar placed in each beaker. Each beaker was labeled with DI water‚ or the pH buffer that was going to be put in the beaker. 50 mL of each of the 5 assigned pH buffers and DI water were obtained using a graduated cylinder and placed in the corresponding beaker. Each of the beakers containing the pH buffer and DI water were placed on the stir plate and the stir plate turned on. A sample of salicylic acid was obtained in a weigh boat and taken back to the lab
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Chemical and Physical Processes of Digestion exercise T 8 he digestive system is a physiological marvel‚ composed of finely orchestrated chemical and physical activities. The food we ingest must be broken down to its molecular form for us to get the nutrients we need‚ and digestion involves a complex sequence of mechanical and chemical processes designed to achieve this goal as efficiently as possible. As food passes through the gastrointestinal tract‚ it is progressively broken down
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This article was downloaded by: [146.83.123.2] On: 15 May 2014‚ At: 14:05 Publisher: Institute for Operations Research and the Management Sciences (INFORMS) INFORMS is located in Maryland‚ USA Interfaces Publication details‚ including instructions for authors and subscription information: http://pubsonline.informs.org Improving Car Body Production at PSA Peugeot Citroën Alain Patchong‚ Thierry Lemoine‚ Gilles Kern‚ To cite this article: Alain Patchong‚ Thierry Lemoine‚ Gilles Kern
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DISCUSSION In this experiment‚ we will extract plasmid DNA that carrying pKan and pAmp from E. coli that have been cultured overnight in LB media containing antibiotic kanamycin and ampicillin respectively. So‚ the DNA that will be introduced later will make the bacteria resistance to antibiotic kanamycin or ampicillin. A plasmid is a small‚ circular‚ double stranded DNA molecules and cloning vector that are widely used for recombinant DNA technology. It can be physically separated from chromosomal
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