In order to carry out a detailed stability study of OXP and CYPZ in combination‚ it is essential to have a single stability-indicating assay method which can separate both drugs along with all of their degradation products from each other. Therefore‚ a RP-HPLC method was developed and optimized. Optimization of the chromatographic conditions In this study‚ the different experimental parameters were carefully studied and optimized in order to improve the performance and system suitability parameters
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vulnerability known as a buffer overflow. It would
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further steps can be taken to increase the yield. Materials and Methods Cell Lysis and Extraction of LDH: Approximately 40 g of minced chicken breast meat (40.327 g) is blended with 75ml cold extraction buffer in four 30-seconds bursts for homogenation of the muscle tissue. The extraction buffer contained 10mM Tris-HCl (pH-7.4)‚ 1mM 2-Mercaptoethanol‚ 1mM Phenylmethylsulfonylflouride (PMSF)‚ 1mM Ethylene diamine tetraacetic acid (EDTA). The homogenization procedure was carried out in the cold room
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Next‚ transferred 200 uL of the crude extract to a set of microcentrifuge tube; the blank is created by adding 200 uL of the homogenizing buffer. Then 800 uL of pre-filtered dye reagent was added to each tube and vortexed for additional 10 seconds‚ followed by an incubation period of 10 minutes (Course Supplement for Bio 101‚ p. 71). We transferred 500 uL of the solution to the cuvette and
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ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? 2. [8pts]Describe the purpose of each loading buffer ingredient added to protein samples for SDS-PAGE analysis (hint- there are 4 ingredients). 3. [45pts]You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls
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liquid nitrogen until the root tips turn to fine powder form. Next is the degradation of RNA step by adding 400 µl Buffer AP1 RNase to the sample in eppendorf tube. The mixture is incubated at 65°C for 10 minutes period after being vortex occasionally. In this period of time‚ the tube will be inverted 2-3 times during incubation. Then lysis step will take over by adding 130 µL Buffer P3. The solution then will be incubated for 5 minutes on ice until the tissue are completely lysed. The lysate solution
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Optical Communication Concept R K Gangwar Divisional Engineer(Transmission) BSNL For circulation to Trainees only Agenda • History • Why Optical Fiber? • Applications of Optical Fiber • Transmission Sequence • Geometry of Fiber • Principle of Propagation of Light • Types of Optical Fiber For circulation to Trainees only History • • • • 1960: Laser invented 1967: New Communications medium: cladded fiber 1960s: Extremely lossy fiber: more than 1000 dB /km 1970‚ Corning Glass
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Bita Heydari Lab report 3 The Effects of Differentiation on Enzymatic Activity Introduction HL-60 cells are capable of undergoing differentiation to induce different cell types. HL-60 cells can undergo morphological changes‚ changes in gene expression‚ and changes in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe
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Student Misconceptions • • • • • Students often believe that the pH at the equivalence point for any titration is 7.00. In terms of problem-solving skills‚ this is probably the most difficult chapter for most students. Students tend to find buffers particularly difficult to understand. Students often forget to consider volume changes that occur when two solutions are mixed (this will have an effect on the concentration of the species present). Students tend to confuse Ksp and solubility.
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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