Introduction The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚
Premium Gel electrophoresis Molecular biology DNA
Effect of pH of the reaction: The impact of the pH of the reaction on the activity of the pure pectinase was studied. The pH range of 3.5to 8 was used (acetate buffer‚ 0.1M; phosphate buffer‚ 0.1M).all the other reaction conditions were at their optimum values as determined from the previous experiments. Table 27: effect of the reaction temperature on the activity of the purified pectinase obtained from Brevibacillus borstelensis
Premium
affect project goals. In the triple-constraints (time‚ cost‚ scope)‚ time remained the most impacting constraint to projects. The critical chain resolved this issue by removing protection time from tasks‚ aggregating them into feeding and project buffers‚ and placing them in strategic locations within the project network where they will serve to protect the project as a whole. This way‚ the fixed elements of each task is the aggressive but possible estimate‚ whilst the variable components are called
Premium Project management Theory of Constraints Management
` PORTABLE MOBILE CHARGER USING PIEZOELECTRIC ENERGY DEEPAK SAHADEVAN NAIR PRADEEP KARUNAMBARAN SUBMITTED ON: 07/JUNE/2013 CIRCUIT DESIGN PROJECT REPORT 7514ENG PRACTICAL ELECTRONICS Griffith University‚ Brisbane‚ Qld‚ Australia EXECUTIVE SUMMARY Alternative energy sources are one of the main focuses in research and development in many aspects in engineering‚ especially electronic devices. By targeting renewable energy sources‚ not only will the amount of pollution decrease but also
Premium Direct current Alternating current Rectifier
BIOL2220 Lab 3: Hill Reaction Introduction In 1937‚ Robert Hill discovered that isolated chloroplasts can generate oxygen when they are illuminated in the presence of a suitable electron acceptor‚ even in the absence of carbon dioxide. This finding was a landmark in the study of photosynthesis because it established that the source of the electrons used in the light reactions is water. It also confirmed that the released oxygen is derived from water instead of carbon dioxide. In chloroplasts
Premium Photosynthesis
east main cofferdam from erosion and damage. Moreover‚ there is a buffer dam which act as a buffer zone. When the wave are very strong that the T-dollos cannot prevent the water going into the dam and the ditches and the holes cannot drain the sea water away‚ the buffer dam is to trap the water in order to protect the east main cofferdam from damaging. If there are no these coastal management such as T-dollos‚ ditches and buffer dam‚ the
Premium Ocean Wind Marine debris
Lexical Analysis Made with OpenOffice.org 1 The role of lexical analyzer: analyzer: It is the first phase of a compiler. compiler. Its main task is to read the input characters & produce as output a sequence of tokens that the parser uses for syntax analysis. analysis. Upon receiving a “get next token” command from the parser‚ the lexical analyzer reads input characters until it can identify the next token. token. It may also perform certain secondary tasks at the user interface. interface
Premium Programming language
Complexometric Determination of Water Hardness 9/12/2013 Abstract: Two sets of acid-base neutralization titrations were conducted for experimental analysis. The first set of titrations was to standardize a solution manufactured in the lab. An approximate solution of Na2EDTA of 0.004 M was titrated against a known solution of 1.000 g CaCO3/L to deter mine to exact molarity of the Na2EDTA. Ca2+ + Na2EDTA → CaEDTA + 2Na+ The second set of titrations was to use the now standardized Na2EDTA
Premium Titration Acid Water
Fluid‚ Electrolyte‚ and Acid-Base Balance: Introduction to Body Fluids 1. a. Where are fluids absorbed? ____________________ b. Where are excess fluids and electrolytes lost?____________________ 2. Name four of the six functions of water. a. b. c. d. 3. a. The amount of water in the body depends on the amount of ________________. b. From the CD‚ list the person with the highest and lowest percentage of water and give the percentage. 1. Highest ___________________ _______% 2. Lowest
Premium PH Blood
above or below a certain value may cause a cell distress or may even be fatal. To help maintain relatively constant pH levels‚ living systems use buffers. A buffer is a combination of a weak acid and a weak base that function together to minimize changes in the pH of a solution. In today’s lab‚ we will learn how to measure pH and demonstrate how buffers work. II) Procedure A) Carbohydrates The basic building blocks for carbohydrates are sugars‚ also called monosaccharides. These molecules are often
Premium PH