Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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the SDS-PAGE and Simple-PAGE gels were prepared with 3.25mL water‚ 2.0mL 4X resolving buffer‚ 2.7mL 40% acrylamide solution‚ 80μL 10% APS‚ and 3μL TEMED. The stacking layers for the SDS-PAGE and Simple-PAGE gels were prepared with 4.6mL water‚ 2.0mL 4X stacking buffer‚ 1.3mL 40% acrylamide solution‚ 48μL 10% APS‚ and 5μL TEMED. The SDS-PAGE gel used the buffers containing SDS and the Simple-PAGE gels used the buffers without SDS. The gels were run at 100 V for about 60
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Presentation Transcript 1. Chapter 17: Recovery System * Failure Classification * Storage Structure * Recovery and Atomicity * Log-Based Recovery * Shadow Paging * Recovery With Concurrent Transactions * Buffer Management * Failure with Loss of Nonvolatile Storage * Advanced Recovery Techniques * ARIES Recovery Algorithm * Remote Backup Systems 2. Failure Classification * Transaction failure : * Logical errors
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HYPER-THREADING TECHNOLOGY 1. INTRODUCTION This report describes the Hyper-Threading Technology architecture‚ and discusses the microarchitecture details of Intel’s first implementation on the Intel Xeon processor family. For that reason‚ firstly‚ general processor microarchitecture and thread level parallelism will be explained. After that‚ hyper-threading technology architecture will be discussed in a detailed manner. Then‚ first implementation examples will be given. Also‚ some important
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To do this‚ following instructions on page 104 in the Microbiology Lab Manual‚ molten agarose was poured into a casting tray where it remained for solidification. Once solidified‚ 300 ml of a buffer was added‚ this is so the electrical field has something to pass through. Next‚ each person pipetted 5 microliters from their PCR tubes into the wells inside the gel. Once all the samples were pipetted‚ the electrical current was added and remained
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Preface‚ Contents 1 Part I Introduction SIMATIC HMI Part II Basic Functions 2 3 10 11 Part III Expanded‚ Configurable Functions OP7‚ OP17 Operator Panel Equipment Manual 13 14 Part IV Commissioning and Description of Devices 16 A Part V Appendix F Glossary‚ Index 6AV3991–1AE05–1AB0 Release 04/99 Safety Guidelines This manual contains notices which you should observe to ensure your own personal safety‚ as well as to protect the product and connected equipment
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To begin the appropriate weight of agarose is weighed out and added to a specific concentration of buffer‚ this mixture is then heated to ensure that all of the agarose polysaccharide is dissolved into the buffer. Once the agarose and buffer are completely mixed they are cooled to room temperature. Dyes such as ethidium bromide can then be added in order for the bands created when the current is applied to be visualized
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Intensive and Critical Care Nursing (2008) 24‚ 28—40 ORIGINAL ARTICLE Pathophysiology of acid base balance: The theory practice relationship Sharon L. Edwards ∗ Buckinghamshire Chilterns University College‚ Chalfont Campus‚ Newland Park‚ Gorelands Lane‚ Chalfont St. Giles‚ Buckinghamshire HP8 4AD‚ United Kingdom Accepted 13 May 2007 KEYWORDS Acid base balance; Arterial blood gases; Acidosis; Alkalosis Summary There are many disorders/diseases that lead to changes in acid base balance
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layer provides the following functions. - Establishing and terminating a logical link between two computers identified by their unique network interface card. - Controlling frame flow by instructing the transmitting computer not to transmit frame buffers - Sequentially transmitting and receiving frames - Providing and expecting frame-acknowledgment‚ and detecting and recovering
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PER 368 – Exercise Physiology Name ____Maggie Hoyt_____ Homework Assignment Chapter 08: Cardiorespiratory Responses to Acute Exercise 1. List 6 components of the cardiorespiratory system that occur during acute exercise. 1) Heart Rate 2) Stroke Volume 3) Cardiac output 4) Blood Pressure 5) Blood Flow 6) The Blood 2. As heart rate INCREASES or decreases‚ the intensity of exercise INCREASES or decreases. 3. Define maximum heart rate (HRmax). The Highest HR value achieved in an all-out
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