The Amplification DNA Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method
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Extraction of caffeine from tea bags ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer was dried
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Liquid CO2 Extraction of D-Limonene This convenient‚ effective and inexpensive liquid CO2-based extraction of a natural product (D-limonene) provides an engaging example of natural products extraction using an alternative solvent (liquefied CO2). Because the procedure can be carried out in a polypropylene centrifuge tube and uses crushed dry ice as its CO2 source‚ it provides an inexpensive alternative to the equipment-intensive extraction with supercritical CO2. A short video clip is available
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of alkaloid caffeine from tea leaves Date Submitted: September 3‚ 2014 Abstract Caffeine is one of the main constituents of tea leaves. In this experiment‚ 0.0039 g of pure caffeine was isolated from tea leaves. This was done through solid-liquid extraction followed by multiple liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value
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nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants in this experiment include‚ the shape and size of the coffee filter‚ the amount of extraction buffer used‚ and the amount of isopropyl
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Abstract: Caffeine‚ (C8 H10 N4 O2) a white‚ crystalline alkaloid known as a nervous system stimulant found commonly in tea leaves and coffee‚ can be extracted using the method of sublimation with the use of Methylene Chloride (CH2Cl2) and Sodium Hydroxide; both can repeatedly rinse the green (unroasted) coffee beans from the caffeine. Keywords: Caffeine‚ Methylene Chloride‚ Sodium Hydroxide‚ Anhydrous Sodium Sulfate I. Introduction Caffeine is an alkaloid of the methylxanthine family. Its chemical
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Extraction of caffeine from Lipton tea leaves Maria Gianna Beatrice L. Cancio*‚ Joe Mari Isabella B. Caringal‚ Rowena A. Chiang‚ Patricia Deanne del Valle Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España‚ Manila‚ Philippines Abstract Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three
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EXTRACTION OF CAFFEINE FROM TEA LEAVES ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer
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Introduction Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. Extraction is a method used to purify a substance by removing the impurities that was added to it. A solvent- solvent extraction is a type of extraction that is most commonly done by using two substances that are typically insoluble with each other. An acid-base extraction is a solvent-solvent extraction‚ in almost every case one of the solvents is water
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Extraction of Caffeine from Tea Leaves Marc Tugaoen‚ Kristine Vanzuela‚ Rafael Villanueva‚ Justeen Wong Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment has been divided into 4 set-ups‚ first was the solid-liquid extraction‚ next was the liquid-liquid extraction‚ then the sublimation and last was the melting point determination. The solid-liquid and liquid-liquid extraction were both done during the first meeting‚ the DCM layer was filtered and
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