In this report‚ the concentration of total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol
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SOLUTION AND CONCENTRATION A solution is a homogeneous mixture‚ made up of a solute dissolved in a solvent. Ex. In a water (aqueous) solution of sodium chloride‚ the sodium chloride is the solute and the water is the solvent. Solute – the component that is dissolved or is the least abundant component in the solution. Ex. Sugar‚ salt Solvent – the dissolving agent or the most abundant component in the solution. Ex. Water Common Types of Solution State of Solution State of Solute
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BTEC Chemistry – Titration experiment I – Standard Solution Prep Preparation of a standard solution of sodium carbonate Anhydrous sodium carbonate is a suitable chemical (primary standard) for the preparation of a standard solution. Standard Solutions are critical in chemistry because you need to have solutions with an EXACT known concentration and volume. Chemicals/Materials: solid sodium carbonate (Na2CO3) distilled water Apparatus: 250cm3 volumetric flask 250cm3 beaker Glass
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Determination of Unknown Acid KEYWORDS: quantitative analysis‚ titration‚ buret‚ endpoint‚ standardization‚ half-equivalence point‚ calorimetric titration‚ potentiometric titration ABSTRACT: The concentration of sodium hydroxide was determined by colorimetric titration‚ and the identity of an unknown acid was determined by potentiometric titration. In the first titration‚ a strong acid standard‚ potassium hydrogen phthalate (KHP)‚ was used‚ to determine the concentration of a strong base‚ sodium
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An Experiment on Soil Analysis Jacob Pitcher and Derrick Gillespie Chemistry Lab 112 Section 02 4/02/2013 Abstract A sample of soil was tested for possible contaminants and other information using a variety of techniques including moisture analysis‚ pH measurement‚ acid-base titration‚ chloride-ion electrode analysis‚ and flame testing. The sample had a greater concentration of chloride ions‚ no measurable CaCo3 ‚ and lower than standard moisture content. It was contaminated with KCl and
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Acid –Base Titration: A volume of 128 mL of 0.650 M Ba(OH)2 was required to completely neutralize 50.0 mL of nitric acid solution (HNO3). What is the concentration of the acid solution? Gravimetric Titration: Calculate the molar concentration of Ba2+ ions in a 500.0 mL sample of an unknown aqueous solution if 2.47 g BaSO4 is formed upon the addition of excess Na2SO4? Stoichiometry of Acid –Base Reactions: What volume of 0.800 M HCl is required to completely neutralize 35.0 g of Ca(OH)2
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Plan a procedure that will allow you to compare quantitavely the glucose concentration in fresh samples of orange‚ lemon and grapefruit juice. Aim: The aim of this investigation is to produce a set of data which will enable comparison of the glucose concentrations of orange‚ lemon and grapefruit juice. Research: Benedict’s Reagent is a mixture of sodium compounds and copper sulphate. The copper ions are reduced by fructose (a reducing sugar) to form copper oxide‚ thus turning it from blue
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Studying the pH of Strong Acid‚ Weak Acid‚ Salt‚ and Buffer Solutions The purpose of the current experiment was to determine the pH of various hydrochloric acid and acetic acid solutions‚ to determine the pH of various salt solutions‚ to prepare a buffer solution‚ and determine the effects of adding a strong acid and strong base to the buffer solution versus adding a strong acid and strong base to water. The measured pHs for the hydrochloric acid solutions were 1.6‚ 2.2‚ 2.9‚ and 3.8. The measured
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Niacin (vit. B3) 0.282 mg (2%) Pantothenic acid (B5) 0.25 mg (5%) Vitamin B6 0.06 mg (5%) Folate (vit. B9) 30 μg (8%) Choline 8.4 mg (2%) Vitamin C 53.2 mg (64%) Vitamin E 0.18 mg (1%) Calcium 40 mg (4%) Iron 0.1 mg (1%) Magnesium 10 mg (3%) Manganese 0.025 mg (1%) Phosphorus 14 mg (2%) Potassium 181 mg (4%) Zinc 0.07 mg (1%) Since orange is a citrus fruit‚ it has 0.005 mol/L citric acid. Citric acid is a weak organic acid with the formula C6H8O7. It is a natural preservative/conservative
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Title of Practical: The Effect of Concentration on Reaction Rate Aim: To use a simple reaction between sodium thiosulphate and hydrochloric acid to discover concentration this determines how fast chemical reactions occur. Independent Variable: Concentration of hydrochloric acid (%) Dependent Variable: Time taken for chemical reaction to take place (sec) Hypothesis: My prediction is that the increased concentration of the thiosulfate will in turn lead to an increase in the rate of reaction
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