ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the
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Chromatography is the separation of compounds to allow us to see the different colours of that compound‚ for instance we may use chromatography in a school lab to see the real colour compounds of plants and grass‚ by using chemicals such as acetone we it will allow the object to break compounds and thus we can see the true colour of the grass. In today’s society there are many real world applications which use the chromatography technique in their line of work‚ an example of one of these applications
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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5.05 Ink Chromatography Basic Lab Components Background Information and Research: 1. Paper chromatography type of method that is used to separate mixtures of substances from a solution. 2. There are many uses for paper chromatography‚ especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood‚ ink‚ or drugs. However‚ the more common use is to
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A eutectic point of 45 °C at 52% Naphthalene was determined from the class data. An unknown crystalline substance was identified to be
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Gather all of your equipment‚ a LabQuest 2‚ two friction blocks (one with sandpaper and one with foam)‚ a force sensor‚ slotted masses‚ a ruler‚ and two sheets of graph paper. Weigh the two friction blocks in kilograms. Then multiply that number by g‚ 9.8‚ to find the blocks’ weights in Newtons and record it. Plug the force sensor into channel 1 of your LabQuest 2‚ this allows you to calculate the amount of force that you pull the block with. Choose six of the slotted masses to place on the blocks
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Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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