Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
Premium Oxygen Photosynthesis Chlorophyll
Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter
Free Solubility Chemical substance Sodium chloride
Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
Premium Food coloring E number Dye
The goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used
Premium Chromatography High performance liquid chromatography Chemistry
of the specialized underlying structures of these life-forms. In order for us to appreciate these special adaptation‚ we first need to know how a typical plant or an animal cell organelle behaves in different water and solute concentrations. In this lab‚ we will determine the effects of hypertonic‚ isotonic and hypotonic solutions on plant and animal cells. In general when an animals cell’s placed in hypertonic solution it shrivels; a plant cell on the other hand undergoes plasmolysis. When an animal
Premium Eukaryote Photosynthesis Cell
“Need a moment? Grab a Twix!” We all know this famous line from every Twix candy bar commercial. The question “Need a moment?” as a general question can refer to a variety of things‚ ever realize though‚ that in every Twix commercial that the ‘needed moment’ is a moment for the men to find a cover story to save their ass? And how after ‘taking a moment’ to eat a Twix they come up with a believable excuse that every woman accepts without question. If you’ve never thought about that before you now
Premium Television advertisement Man Woman
In the novel‚ Of Mice and Men‚ by John Steinbeck‚ the connection between Candy and his dog is very similar to the relationship between George and‚ Lennie. Candy and George are both caretakers; they look after their partners‚ even if they don’t fit in. The old dog is blind past his prime‚ and Lennie is naive and childlike in nature. It is not their fault that they are who they are because they don’t know any different. Candy defends his dog when the other workers try to convince him to get rid of
Premium KILL Of Mice and Men John Steinbeck
blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
Premium Photosynthesis Oxygen Carbon dioxide
Salinity is the measure and concentration of all the salts dissolved in water. Usually measured in parts per thousand (ppt or )‚ ocean salinity is 35ppt and the average river water salinity is 0.5ppt or less (University of Rhode Island). Many factors go into salinity‚ making water salty. To name a few‚ salinity affects the environment through sediment from rocks washes into the ocean‚ evaporation of ocean water‚ and sea ice formation are known to increase the salinity of the ocean (NASA). It
Premium Sodium chloride
Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
Premium Germination Seed Embryo