Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent
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Childhood obesity is a serious medical condition that happens when a child’s weight is well above normal standards for his or her age and height and it has become an ever-increasing problem in the last three decades. Between 1976-1980 and 2007-2008‚ pre-school aged children 2-5 years increased in obesity from 5 to 10.4%. Among 6-11 year olds‚ that rate increased from 6.5 to 19.6% and from 5 to 18.1% among adolescents aged 12-19. (Ogden & Carroll‚ 2010‚ para. 3). Childhood obesity is particularly
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance‚ followed
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We will be testing these plants molecular and structural traits to see which one is closely related to the Botana Curus ‚ using microscopes simulated electrophoresis and much more. MATERIALS: The materials we used : 1) The Lab packet 2) Foam cups 3) Chromatography paper 4) Pen or Pencil 5) Microscope slides for species x ‚y ‚z ‚and the Botana Curus 6) Microtip dropper 7) Plant extract 8) Microscope 9) H2O Procedures:
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itself and George can no longer keep that belief. By shooting Lennie‚ George spares his friend the painful death that would be given by Curley’s lynch mob‚ but he also puts to rest his own dream of a perfect world. Candy The old handyman‚ aging and left with only one hand as the result of an accident‚ worries that the boss will soon declare him useless and demand that he leave the ranch. Of course‚ life on the ranch—especially Candy’s dog‚ once an impressive sheep herder but now toothless‚ foul-smelling
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Objective: The objective of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of
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Briefing Thin-layer chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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can hardly tell‚ that it isn’t an alcoholic beverage. With an awesome drink recipe I’m going to show you today‚ no one can even guess that you’re drinking a non-alcoholic beverage. If you’re hosting a baby shower or meet the baby party‚ this Cotton Candy Raspberry Cream Mimosa would be a wonderful choice. The cocktail is very festive‚ with fresh berries and lots of bubbles. One last thing I’d like to mention. Since we’re talking about wine‚ that went through a process of de-alcoholization‚ there is
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