Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Separating Water Soluble and Acetone Soluble Inks through Chromatography Abstract: Chromatography is a term used to define a set of laboratory techniques for the separation of mixtures. Through Chromatography we analyzed complex mixtures. In our recent lab we analyzed complex mixtures. The purpose of this lab was to find whether the water soluble or acetone soluble liquid traveled farther. In our recent lab we measured the distance traveled by the different colored water and acetone soluble
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The “Chromatography Instrumentation Market by Systems [LC (HPLC‚ UHPLC‚ FLASH)‚ GC‚ TLC‚ SFC‚ Others (Autosamplers‚ Detectors‚ Fraction collectors)]‚ By Consumables (Reverse phase columns‚ Syringe filters‚ Vials) & Industry - Global Forecast to 2018"‚analyzes and studies the major market drivers‚ restraints‚ opportunities‚ and challenges in North America‚ Europe‚ Asia‚ and Rest of the World. Browse 123 market data tables and 49 figures spread through 407 pages and in-depth TOC on Chromatography
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Thin-Layer Chromatography of Analgesics Abstract: In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC‚ ASP‚ CAF‚ IBU‚ SAL‚ an unknown consisting of a mixture of three of the references‚ and two unknowns (6‚23) containing a single reference compound. The composition of the unknown mixture and two single unknowns were determined by spotting the reference compounds and the unknown compounds on two
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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CHAPTER 4 RESULTS AND DISCUSSION 4.1 Identification of camphor and menthol using gas chromatography – mass spectrometry (GC – MS) In this study‚ the presence of menthol and camphor were determined from 5 different locally produced medicinal topical ointment samples. Sample of Vick® VapoRub® and Hurix’s Massage Balm were purchased from local pharmacies‚ Bunga Cengkih Balm and Serai Balm samples were purchased from the grocery shop. Buah Pala Balm sample was purchased from the night market
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Experiment 5 Analysis of Analgesic Tablet by High Performance Liquid Chromatography Abstract An unknown sample‚ 529‚ was tested using high performance liquid chromatography to detect the concentrations of acetaminophen‚ aspirin‚ and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen‚ 11.5±0.185mg/100mL of aspirin‚ and 4.89±0.185mg/100mL of caffeine. Based on accepted values‚ the maximum daily amounts of each compound are 4000mg of acetaminophen
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goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used in
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Drugs screen using Thin Layer Chromatography of basic illicit drugs Abstract The majority of evidence submitted to crime labs comes from drug-related crimes. Often‚ this evidence includes unidentified powders that may be illegal drugs. In order to prosecute individuals for possession of illegal substances‚ it is necessary for forensic scientists to positively identify any suspected drugs submitted to the laboratory. In addition‚ forensic toxicologists must determine the identity
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Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
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