exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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3.7 Nuclear Independent Chemical Shift Analysis The Nuclear Independent Chemical Shift is measured as magnetic shielding of a molecule have been calculated at the B3LYP/6-31G theory level. Aromaticities are represented by negative NICS Values and antiaromaticity is represented by positive NICS Values. The large negative NICS Values of the title compound represent stronger aromaticity [29-35].Our present investigation of the density functional calculations of doped fullerene with glycine shows several
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Objective: The goal of the experiment was to use UV/VIS spectroscopy to collect data to quantify acetaminophen‚ aspirin‚ and caffeine from a migraine tablet where there is heavy spectral interference using Target Factor Analysis1. The migraine tablet has other ingredients in it like FD&C blue #1‚ stearic acid‚ and saccharin. Saccharin is the ingredient thought to have the biggest interference and was used as the experimental basis2. Instruments: UV/VIS spectroscopy was used to analyze the absorption
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(1) Answer to Question in lab manual. NO introduction/background etc. 1. What is the retention time for caffeine? Retention time for caffeine : 4.149+4.142+4.152+4.1454 = 4.15 2. What is the retention time for benzoic acid? Retention time for caffeine : 2.651+2.817+2.857+2.9274 = 2.81 3. How are the retention times for caffeine and benzoic acid related to their chemical structure and the nature of the chromatographic
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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corresponding anthocyanidins using paper chromatography‚ and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g‚ followed by blackberries at 1.19 x 10-6 g‚ finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins‚ since it contained the most streaks on the chromatography paper. The main conclusion that can be
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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5.05 Ink Chromatography Basic Lab Components Background Information and Research: 1. Paper chromatography type of method that is used to separate mixtures of substances from a solution. 2. There are many uses for paper chromatography‚ especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood‚ ink‚ or drugs. However‚ the more common use is to
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Towers Analia’s mother died of a delirious fever when she was born and her father could not bear the sadness and two weeks later there was a pistol shot in the chest. He agonized for several days with his wife’s name on his lips. His brother Eugenio administered lands and ordered the family destination of the little orphan at its discretion. Until six years Analia clinging to the skirts grew an Indian loves the service rooms of the house of his tutor and later‚ aged just had to go to school‚ was
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