January 27‚ 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second‚ each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen‚ Aspirin and Caffeine. Finally‚ calculating the Rf value by measuring the mobile and stationary phase. Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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Haldiram’s From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search [pic] |[pic] |This article may require cleanup to meet Wikipedia’s quality standards. Please improve this article if you can. (March | | |2010) | Haldiram’s started in Bikaner‚ India is one of India’s largest sweets and snacks manufacturers. Haldiram has its establishment in many parts
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Introduction Kit Kat is known by everyone for its tasty chocolate wafers but also for its “have a break”. Kit Kat‚ Kit Kat Chunky or Kit Kat balls are the most famous products in the French market‚ but the bars of chocolate exist all over the world in more than 70 countries. Here are some key facts: * The Kit Kat range‚ which is produced in York‚ makes up approximately 23% of Nestlé Confectionery UK sales. Almost a quarter of the Kit Kat sales are of four–finger Kit Kat. * The UK is the
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Introduction: The purpose of this experiment is to use sodium borohydride to reduce benzil. However‚ stereochemistry allows for five possible products. If only one carbonyl group is reduced during the reaction a racemic mixture of benzoin will be the product that is produced. After the first reduction a chiral center forms causing the second reduction to occur from only one side of the ketone. Depending on which side the second reduction take place there are three possible products including: a racemic
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Subject: Thin-Layer Chromatographic Analysis of Drug Components (Experiment #15) The purpose of this experiment is to determine the components of an unknown drug and identify it as one of six (6) commercial drugs by using thin-layer chromatography. I added approximately a quarter of a tablet of acetaminophen‚ aspirin‚ caffeine‚ ibuprofen‚ salicylamide‚ and the unknown (#19) to separate test tubes containing 2.5mL of dichloromethane. I noticed that my unknown was a coated tablet.
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The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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usually masked by the prevalence of the green chlorophyll. Anothocyanin(red or purple)‚ carotene(orange)‚ and xanthrophyll(yellow) are found in different proportions in different leaves. Leaf pigments can be separated by using paper chromatography. Paper chromatography is a technique that extracts pigments into a paper filter called chromatogram. What are the pigments are in Red Wondering Jews? Carotenoids These pigments primarily absorb in the blue wavelengths‚ allowing the longer wavelengths
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Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
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