Fractional Distillation & Gas Chromatography Exp. 1 Pre-Lab: 1) When two substances whose molecules are very similar from a liquid solution‚ the vapor pressure of the mixture related to vapor pressure of the pure substance. Also it could be defined as a two liquid are ideal solution when they don’t react with each other and they make no association. 2) Are a mixture of at least two different liquid‚ and known also as a mixture of two or more liquid in such away that its component
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Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
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experiment is to identify an unknown proprietary drug using thin-layer chromatography. The unknown’s behavior in thin-layer chromatography will be compared with that of its possible component analgesics. The possible unknowns and their analgesic ingredients will be Anacin (aspirin‚ caffeine)‚ Excedrin (acetaminophen‚ caffeine‚ aspirin)‚ Motrin (ibuprofen)‚ and Tylenol (acetaminophen). Introduction Thin-layer Chromatography(TLC) was the method used to figure out and identify the drug. TLC is done
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Independent Two Sally Mann captured this photo of her three children naming it "Candy Cigarette". Sally Mann parented her three children‚ and as they grew older‚ but still at a very young age‚ they were either able or not able to handled their own decisions independently. This black and white photograph represents three young children who may or may not be independent. In this photograph‚ the long narrow path is leading to what either could be something good or bad. The white clothing the young
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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Mobile liquid phase is always passed stationary phase during chromatography. Developed TLC plate is then observed under short wave ultraviolet light‚ where most TLC plates contain fluorescent mixed with silica gel that allow to glow green color. Compounds will absorb the UV light and appear as dark spot against the green color TLC plate. In a column chromatography‚ cotton and sand is placed in purpose of holding silica gel and prevent leakage of adsorbent particles
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down the book Gym Candy by Carl Deuker. It is a great book about sports and truly displays the life of a football player and what some people are willing to do to become great. The main character in my book is Mick Johnson and ever since he was three he was striving to be great football player like his father was. But no matter how much effort he displays on the field or in the gym he always comes up short. So Mick decides that it’s time for a change so he decides to use “gym candy” to become bigger
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The Candy Man By : Nadia Guadarrama This is a story about a young girl and how her life is a mess. Our story begins in a small town called Hudson. It was the middle of summer and our young girl named Nadia Guadarrama was moving back to her home town‚ Hudson. She grow up there and loved it there but her famly moved into the city but now they re moving back to their old house upin Hudson. It was June 20 2018 and she had just oved into her home. Nadia was upseted with ghost and belived in them
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12 August 2013 Memory of a Modern Idea In the well-known picture “Candy Cigarette” by Sally Mann‚ the cloudy gaze of a girl is captured in a moment of time. The black and white photo gives off an eerie familiarity that reminds the viewer of a distant memory. The picture offers a clear vision of a girl but also an abstract idea of children in general. This is an interesting piece of art mainly because its purpose was to convey a message‚ it was not just art for the sake of art‚ it was meant
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Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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