Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*‚ Phoebe Abalos‚ Kevin Antiga‚ Carmelus Aseneta 3-Biology 2 College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted
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________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals and then you will identify
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the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other‚ a yellow tint. Therefore‚ based on this qualitative data‚ the dyes in the green Kool-Aid are
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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of wanting to mimic those who are closest to us. Although we all are visually stimulated by what surrounds us and even by our closest family members; children are more vulnerable and sensitive to this kind of stimuli. In Sally Mann’s photograph‚” Candy Cigarette”‚ the common issue that we see in children that is‚ their desires to rush into adulthood
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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