January 1999 B io Factsheet Example glucose fructose galactose Number 39 Carbohydrates: Revision Summary Carbohydrates contain 3 elements: Carbon (C)‚ Hydrogen (H) and Oxygen (O). Thus‚ if we remove water from carbohydrates‚ all that remains is carbon. Carbohydrates can be divided into 3 categories; monosaccharides‚ disaccharides and polysaccharides (Table 1). Table 1. Structure of monosaccharides‚ disaccharides and polysaccharides Category Monosaccharide (made of 1 sugar molecule)
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Chemistry Lab Report on standardization of acid and bases. Purpose: To prepare standardize solution of sodium hydroxide and to determine the concentration of unknown sulfuric acid solution. Data and Calculations: This experiment is divided into two parts (Part A and Part B). In the first part of experiment‚ the standardize solution of sodium hydroxide is prepared by titrating it with base Potassium hydrogen phthalate (KHP). The indicator Phenolphthalein is used to determine that whether titration
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Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify
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Austin Peay State University Department of Chemistry CHEM 1021 IDENTIFYING CARBOHYDRATES (adapted from Blackburn et al.‚ Laboratory Manual to Accompany World of Chemistry‚ 2nd ed.‚ (1996) Saunders College Publishing: Fort Worth) Purpose: To become familiar with some of the characteristic reaction of carbohydrates. To identify an unknown carbohydrate. Caution: Wear eye protection because of the possible shattering of dropped glassware and because acids are used in this experiment. Seliwanoff reagent is resorcinol dissolved in 4M hydrochloric acid
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Lab BCH 2333 Section: Lab 1 Carbohydrates: Separation Techniques Based on Molecular Size TA: Wednesday‚ January 16th‚ 2013 Team #4 By: Partner: Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric
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Anna Powell 2nd I.Title: Acid-Base Titrations AP Chemistry Laboratory #6 II.Purpose: The purpose of this experiment is to standardize a sodium hydroxide solution and use the standard solution to titrate an unknown solid acid. The equivalent mass of the solid acid will be determined from the volume of sodium hydroxide added at the equivalence point. The equilibrium constant‚ Ks‚ of the solid acid will be calculated from the titration curve obtained by plotting the pH of the solution versus
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The Chemistry of Lipids Experiment #8 Objective To observe the solubility of lipids in polar and nonpolar solvents and to compare saturated and unsaturated fats in their chemical reaction with bromine. The percent fat in a food will be determined by extraction of the fat and weighing it. Introduction Lipids‚ by definition‚ are natural substances that do not mix with water but dissolve in organic solvents. There are several classes of lipids‚ including: fatty acids‚ waxes‚ triacylglycerols
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Purpose: To find out if the Unknown substances are bases or acids. Materials: goggles aprons paper for data table graduated cylinder unknown I substance beakers/test tubes stirring rod litmus paper pH indicator paper pH color chart phenolphthalein scoopula eye-dropper digital pH meter magnesium ribbon calculator unknown II substance forceps sodium bicarbonate Procedures: Test the pH of the Unknown substances with litmus paper and pH indicator paper and match to color chart
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Carbohydrates H2SO4 acts as dehydrating agent forming furfural derivatives which interact with alpha-naphthol librating a violet layered compound Test | Molisch test | Objective | A general test for all carbohydrates. Monosaccharides give a rapid positive test. Disaccharides and polysaccharides react slower. | Principle involved | Dehydration of the carbohydrate by concentrated sulfuric acid to produce an aldehyde | Reagent | H2SO4 | Positive results | a purple-black layer/violet ring
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are several purposes to the amino acid lab experiment. One reason is to help us understand the concept of acids and bases and how they interact with one another. Another purpose is to understand what occurs when placed with the existence of buffers. Lastly‚ the experiment is to help us understand the applications of titrations and how it can be used in a real world setting‚ in this case‚ the identification of an amino acid. One must understand that amino acids all have special characteristics
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