=========== BIOCHEMISTRY ============ Biochemistry Laboratory - CH 601L (2011-2012) Experiment 6 Characterization of Saponifiable Lipids Kevin Arven A. Benin*‚ and Gabrielle Marie A. Bugna Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: August 25‚ 2011 ________________________________________________________________________ Abstract:
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reducing sugars o Biuret test checks for protein Notes on Underlying Theory Introduction • The most abundant elements in living material are: o Carbon o Hydrogen o Oxygen o Nitrogen o Sulfur o Phosphorus • There are 4 major types of biological macromolecules: o Carbohydrates • Monosaccharides (i.e. glucose‚ fructose)
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Lipids are made up of carbon‚ hydrogen and oxygen (phospholipids also contain phosphate) and are found in a wide variety of organisms from bacteria to eukaryotes where they perform a wide variety of useful functions. This essay will expand on these functions to describe the role of lipids. There are several types of lipid including triglycerides‚ steroids‚ waxes and phospholipids. Triglycerides are made up of three fatty acids combined with glycerol by an ester linkage meaning they are predominantly
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Carbohydrates For my health project I did carbohydrates. They are the bodies energy source. Carbohydrates supply the body with the energy it needs to function. They are found almost exclusively in plant foods‚ such as fruits‚ vegetables‚ peas‚ and beans. Milk and milk products are the only foods derived from animals that contain a significant amount of carbohydrates. Carbohydrates are divided into two groups‚ simple carbohydrates and complex carbohydrates. Simple carbohydrates‚ sometimes
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stimulation initiates a cephalic-phase metabolic reflex that increases appetite (10). The long-term effects of artificial sweeteners on food intake and body weight are less clear. Although some investigators report weight gain in animals given artificial sweeteners to eat or drink (1 1-13)‚ the majority reports no effects (11‚ 14-17). What little work has been done in humans does little to answer the question. Two correlative comparisons ofusers and nonusers of artificial sweeteners showed that the sweeteners
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Standard Reduction Potentials‚ Equilibrium Constants and Investigating a Lead-Acid Electrolytic Cell Purpose Experimental Methods All procedures were followed according to the lab manual (experiment 10 – Electrochemistry Laboratory). Data and Observations Part A: Grams of FeSO4 used: 0.759 Voltage during voltmeter and battery check: 9.23V Table 1. Electrochemical Cells Data Part B: Table 2. Lead-Acid Battery Results and Calculations In order to make 5ml of 1M FeSO4(aq)
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Weak Acid Titration Abstract: Our method for determining the unknown weak acid was to determine the equilibrium constant K from the molecular weight of the weak acid from our titration data. In this lab the acid Potassium hydrogen phthalate and two unknown acids were titrated. We determined the molar mass of the Potassium hydrogen phthalate‚ for the unknown acids we calculated the molar mass and the Ka values. We used NaOH as the known base for titrating in all three of the titrations. Our
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Where ’s the Protein? Proteins‚ which are constructed from amino acid monomers‚ are considered one of the four major macronutrients needed by the body‚ along with carbohydrates‚ lipids‚ and nucleic acids. Only the first three nutrients listed require delicate balancing. One must consider the sources from which he or she receives those required nutrients. Carbohydrates from fruits‚ vegetables‚ and grains; lipids from oily animals such as fish or plant foods like avocados; and proteins from meats‚
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methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by a PCR and cloned into a vector. This Polycomb group proteins help maintain the cell identity during
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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