#) Identifying Carbohydrates (First inital and last name of lab partner) Purpose The purpose of this lab is to learn how to identify different forms of carbohydrates by conducting the Benedict and Iodine test. Theory The theory for this concept is that if in the benedicts test the carbohydrate reacts‚ it is a monosaccharide. If it reacts in the Iodine test it is a polysaccharide. If no reaction occurs in either test the carbohydrate is a disaccharide. Data Type of carbohydrate | Benedicts Test
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MODULE 2: GENETICS‚ VARIATION AND NATURAL SELECTION SPECIFIC OBJECTIVES & EXPLANATORY NOTES 1. Structure and Roles of Nucleic Acids 1.1 illustrate the structure of RNA and DNA using simple labelled diagrams; The genetic substance found in all organisms called DNA or deoxyribonucleic acid is a kind of nucleic acid. Nucleic acids consist of two long polymers of simpler units‚ called nucleotides; that are composed of three (3) main units as shown below: 1) A pentose sugar (deoxyribose
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Freem an‚ Biological Science‚ 4e‚ Chapter 4 Chapter 4 - Nucleic Acids and the RNA World Learning Objectives: Students should be able to... • Sketch a nucleotide‚ label its three basic parts‚ and identify the 2’‚ 3’‚ and 5’ carbons. • Make another sketch showing the primary and secondary structures of DNA. • Describe the primary‚ secondary‚ tertiary‚ and quaternary structures of RNA‚ and explain in what ways RNA differs from DNA. • Explain why and how the secondary structure of DNA allows
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In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included
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With an improved procedure the question could have been answered with this methodology‚ but the aim was to find out the affect of carbohydrates on blood glucose levels‚ but the methodology provided an answer for the question without going into depth of the biochemistry and carbohydrate structure. This could be solved by amending the aim or by constructing some sort of pre-experiment with the bread that would be used to compare the structure of Danskt Rågbröd
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Bita Heydari Lab report 3 The Effects of Differentiation on Enzymatic Activity Introduction HL-60 cells are capable of undergoing differentiation to induce different cell types. HL-60 cells can undergo morphological changes‚ changes in gene expression‚ and changes in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe
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ISOLATION AND CHARACTERIZATION OF NUCLEIC ACIDS Jan Eric C. Balete‚ Dorinne P. Barretto‚ Divine Trisha Angela T. Batac‚ Neill Steven C. Cachuela‚ Karel D. Cartagena Group 2 2C Medical Technology Biochemistry Laboratory ABSTRACT fgdfgdgdfgfdfgd INTRODUCTION Nucleic acids are informational molecules with their primary structure containing a code or set of directions by which they can duplicate themselves and guide the synthesis of proteins. [1] They are very large molecules built from subunits
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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To purify the protein in the cell lysate from lab 1 through nickel affinity chromatography. Protein purification should result in only one type of protein ideally‚ which is the protein of interest‚ wt-DHFR and mut-DHFR in this case. A pure protein allows for further analysis on the protein to be conducted‚ such as its concentration (Bradford assay)‚ its molecular weight‚ and its biological activity. 2. Overview of experiments Buffer Preparation Add the liquid sodium phosphate‚ solid sodium chloride
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Task 1 • Describe the structure of an enzyme as a protein‚ in terms of tertiary/ quaternary structures. 1) Primary Structure This is in reference to the order of way that amino acids are connected to form a protein. These are built up from 20 amino acids‚ and follow these structures o A carbon (the alpha carbon) bonded to the four groups below: o A hydrogen atom (H) o A Carboxyl group (-COOH) o An Amino group (-NH2) o A "variable" group or "R" group 2) Secondary Structure This is in reference
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