Lab BCH 2333 Section: Lab 1 Carbohydrates: Separation Techniques Based on Molecular Size TA: Wednesday‚ January 16th‚ 2013 Team #4 By: Partner: Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric
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that is vital is protein. Protein is one of four major building materials in the human body. Proteins are composed of carbon with Hydrogen‚ Oxygen‚ and Nitrogen attached. Some proteins also have Sulfur‚ Phosphorus‚ and Iron. Proteins are made up of sub-units called amino acids‚ of which there are twenty. These are just the basics of what proteins are composed of. Amino acids‚ which are the sub-units of Proteins‚ can be divided into two groups. Eleven of the twenty amino acids can be made in the
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EXPERIMENT NO. 6 LIPIDS I NUR 4 GROUP 9 POLICARPIO‚ JOHN PAUL (A & B) QUERUBIN‚ KIMBERLY (C & D) QUILALA‚ REGENE (E & F1) RAMOS‚ PRACCEDES (F2a & F2b) SABALDICCA‚ VENZ (F2c & F2d) INTRODUCTION Lipids are organic compounds found in living organisms that are insoluble or slightly soluble in water but soluble in non-polar organic solvents. Lipids can be classified into four groups which are -fats‚ oils‚ and waxes‚ -compound lipids‚ -steroids‚ and -derived lipids. Various experiments
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Title: Estimation of Ascorbic acid Aim: To determine the ascorbic acid content of canned apple and orange juice To determine the volume of canned juice necessary to provide 75mg of Vitamin C Introduction: For the estimation of ascorbic acid‚ the method used involved the titration of the ascorbic acid‚ in the presence of a redox indicator 2‚6-dichlorophenol-indophenol (DCPIP) which acts as both an oxidant so as to oxidise the ascorbic acid to dehydroascorbic acid‚ and it acts as an indicator
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(Fe3+ ) form. Ferric form is not usually absorbed in the body. In the stomach‚ there is low pH of 2 due to presence of gastric acids such as Hydrochloric and Ascorbic acids. These acids provide an acidic environment for reduction of ferric iron to ferrous (Fe2+). Ferrous iron can be absorbed in the body system. As in following equation of iron (iii) reaction with Ascorbic acid ‚ the product is iron (ii) chloride which is soluble 2 Fe3+ (s) + C6 H8 O6 (aq) → 2 Fe2+ (aq) + C6 H6 O6 (aq) + 2 H (aq)+
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AMINO ACIDS BY MEANS OF TITRATION CURVE ABSTRACT The aim of the experiment was to identify an unknown amino acid through acid-base titrations which was prepared in water to form an acidic solution. Each group prepared unknown amino acid hydrochloride. The pre-prepared basic solution was slowly added to the amino acid solution and pH change was closely observed using the pH meter. During these titrations the amino acid converted from cation to zwitterion to anion; zwitterion is an amino acid with
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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adopting new technologies and are using feed acidifiers for supplementing the diets of animals. The market value of propionic acid as a feed acidifier was the highest in 2012‚ and is projected to grow with the increasing demand for feed acidifiers in the livestock industry. The benefits of feed acidifiers are leading the market demand in the feed industry. The fumaric acid market is comparatively small and is projected to grow at a CAGR of 5.8% from 2013 to 2018. The feed acidifiers market is projected
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787122 Received 3 October 2002/Accepted 21 November 2002 Downloaded from http://aem.asm.org/ on June 6‚ 2013 by UNIVERSITY OF DELHI Despite their commercial importance‚ there are relatively few facile methods for genomic manipulation of the lactic acid bacteria. Here‚ the lactococcal group II intron‚ Ll.ltrB‚ was targeted to
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ACID BASE IMBALENCE INTRODUCTION The body normally maintains a steady balance between acids‚ produced during metabolism and bases that neutralize and promote the excretion of the acids.Many health problems may lead to acid base imbalance.Patients with Diabetis mellitus ‚COPD‚and kidney disease frequently frequently develop acid base imbalences. Vomiting and diarrhea may also cause acid base imbalance.The kidneys are an essential buffer system for
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