Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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Released in the year 1956‚ “The Minority Report” is a science-fiction short story written by Philip K Dick. Released in the year 2002‚ director Steven Spielberg adapted the short story into a film and released it as Minority Report. Despite having the same central focus (free-will and determinism)‚ there are not only some similarities but also differences in the narrative and style of both. In both the short story and film‚ the author and director respectively use narrative techniques that allow
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ENGL 1301 Final Examination To all exam takers: You must complete the exam in order to pass the class. Write a 3-4-page double-spaced essay to reflect on what you have learned about writing. You are by no means limited to the following questions‚ but writing around these questions can help you analyze your progress as a writer. This is a personal essay‚ so you can use the first-person point of view that shares your true observations in your personal voice. Grading criteria This essay must
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ACC 4043/6043 Governmental Accounting CAFR CASES Instructions: Obtain a copy of a recent Comprehensive Annual Financial Report (CAFR). You are to answer the attached questions for CAFRs 1 to 5. You do not have to do the other ones. In responding to the questions‚ recognize that yes and no answers are generally not sufficient. I am allocating significant points to these cases‚ and I want your answers to indicate that you have read and understood the information presented in your
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ENGL 101 Basic English Universidad Metropolitana‚ Universidad del Este‚ Universidad del Turabo © Ana G. Méndez University System‚ 2004. All rights reserved. Prep. 26.APR.04 Dr. Carmen L. Lamboy Escuela de Estudios Profesionales Programa AHORA Universidad del Este‚ Universidad Metropolitana TABLE OF CONTENTS STUDY GUIDE -------------------------------------------------------------------------------------------------------- 4 WORKSHOP ONE ---------------------------------------------------------------------------------------------------
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Experiment 6: Synthesis of an Alkyl Halide Maria Alexandria Buraga Ammuyutan Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ------------------------------------------------- Department of Food and Science Nutrition‚ College of Home Economics‚ Univeristy of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ------------------------------------------------- ABSTRACT ------------------------------------------------- Alcohols react with hydrogen
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Purpose Use a standardized potassium permanganate solution to analyze an unknown iron solution by using redox titration. Theory Reduction-oxidation titration is an analytical method based on electrons transferring between an oxidizing agent and a reducing agent in solutions. Chemical analysis can be built on Redox titration if four criteria are met: a) The reaction is completed in a thermodynamically spontaneous condition. b) The reaction is fast enough to give an operational result instantly
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SOC/120: Evolution of Formal Organizations Due: 12/11/2011 Nancy Keta The way that I will conduct my research is to first explain how formal organizations have evolved over the past century. Second‚ what differences were there in organizations a century ago‚ and compare to today’s organizations. And lastly I will also tell of the trends in today’s formal organizations in modern society. I will describe how each of the characteristics for formal organizations will need to evolve or change in
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Your Name Here ENGL101-B05 Discussion Board Forum 1 May 17‚ 2013 Hochswender was very Persuasive – Turabian After reading the essay by Woody Hochswender “Did My Car Join Al Qaeda‚” I found that it was very persuasive. Hochswender had persuading support for his point of view. Just because he drives a SUV which uses a lot of gas‚ this doesn’t make him a bad person. Nor does it make him responsible for what is going on in Al Qaeda any more than a taxpayer is responsible for all government spending
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Appreciating Our Real Values The idealized images‚ the pictures of perfection‚ are what we usually see in advertisements nowadays. Commercials have become a great part that we encounter in our daily life; as a consequence‚ we are all affected by the perfect outlook of what showed on mass media. As a case in point‚ Americans are usually attracted by the greatly designed sport utilities vehicles. These vehicles are usually a symbol of an ideological value through the image of a powerful‚ break-through
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