BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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Sometimes some people like to go for vacation to the south carolina in the mountains and some like to go to the beach. Some people like to have a other view so they like to go to florida to the beach for vacations Like to spend time with there family in the mountains and make a fire‚ and camp go for a walk in the woods‚ Some people like to have some time with there family in the beach and build sand castles‚ Go fishing‚ have fun with there family. The purposes of this essay is to compare and contrast
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John Lawson wrote the book “A new Voyage to Carolina” in 1674-1711about the life changing experiences he had with Native Americans and how they impacted his life in many different ways for example he was very fascinated with the art of dancing‚ the way Native Americans danced was very strange yet incredibly amusing. Mr. Lawson saw dances that looked like it was turning into a fight and at the same time they were moving slowly without making any noises at all which he admired a lot. Marriage was a
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Tiffany Galimi History 11 Section 11 The Founding Of the Colony of North Carolina In the 1580’s British established two colonies in North Carolina‚ both in which they failed. However‚ In the 1600s permanent settlers from Virginia began to move to North Carolina‚ and it eventually became part of a British colony known as “Carolina." North Carolina was first settled in 1587 by settlers that were led by John White in which they landed on present day Roanoke Island on July 22‚ 1587. In 1524
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North Carolina is described as an unstable and tumultuous place. It seems to have been since the 1600’s. North Carolina was against high taxes and the Internal Revenue Services. How the government put taxes on the land where the North Carolinians worked so hard to keep their land up. This all occurred when King Charles ll was in office. In the late 1600’s in England the tumultuous political royalist had continued to assist King Charles ll in giving the land to eight of his Proprietors. During
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North Carolina and Rhode Island possess both similarities and differences for founding and development. As a result of geography and previous history‚ North Carolina and Rhode Island vary in their relations with Native Americans. Rhode Island and North Carolina share similarities pertaining to motivations for colonization‚ political development and religious attitudes. Rhode Island was essentially founded by Roger Williams who fled to the area in 1636 with the help of amicable Indians. Rhode
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Racism Against People Of Color In North Carolina Racism is a socially endured/influenced action. It is something that has been happening since the beginning of time and it’s acquired by people of all ages and races; It is a learned behavior‚ passed down from generations to generations. North Carolina is inhabited by a large quantity of people of color‚ Native Americans‚ African Americans‚ Latinos‚ Asians‚ etc. Hence‚ everyone should feel comfortable and accepted. The effects of racism while growing
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HOW NORTH CAROLINA IS EFFECTED ECONOMICALLY BY WATER POLLUTION About 80% of the State’s surveyed freshwater rivers and streams have good water quality that fully supports aquatic life uses‚ 17% have fair water quality that partially supports aquatic life uses‚ and 3% have poor water quality that does not support aquatic life uses. Ten percent of the surveyed rivers do not fully support swimming. The major sources of impairment are agriculture (responsible for 53% of the impaired river
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South Carolina ’s Tax Changes What Will They Mean? Donald L. Schunk Act 388 is not good news‚ either for our pubiic sctioois or for our iong-term tax rates. I n 2006‚ the South Carolina General Assembly passed major legislation affecting both the tax structure and the way public education is funded in South Carolina. The legislation - Act 388 - contains three major components. The first involves raising the state sales tax from 5 percent to 6 percent effective June 1‚ 2007. The
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necessary for digestion of lipids‚ carbohydrates‚ protein? Certain conditions are required for digestion. I will find that enzyme is required for digestion of lipids‚ carbohydrates‚ protein. There are many conditions that are necessary for digestion of lipids‚ carbohydrates‚ protein such as pH and enzyme and substrate. The test tube that digestion of lipids occurs in was test tube 1‚3‚4‚ test tube 2 there was no digestion because there was no enzyme. Test tube 1 was the fastest for digestion because the
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