LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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When there are two different concentrations‚ a concentration gradient exists. When things move from a higher concentration to a lower concentration‚ it is known as diffusion. As long as diffusion is permitted to go on‚ equilibrium will be reached. Equilibrium is when two areas have reached equal concentration levels. The egg in this lab will have water and substances diffusing though it in and out. The direction‚ in which things diffuse‚ depends on the type of solution the egg is placed in. A hypertonic
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Osmosis Aim: To determine the concentration of cell sap in a potato cell. Osmosis is the movement of water through a semi-permeable membrane. It starts from a weak solution and becomes a more stronger solution until the concentrations on both sides are equal. To investigate the range of solutions needed to find the point at which the surrounding solution is the same as the cell sap in the vacuole.
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Transpiration Formal Lab Report By Jessica Tran Introduction When water is transported from the roots to the mesophyll cells in the leaves‚ it is evaporates out the stomates‚ called transpiration‚ to create a lower osmotic potential. Osmotic potential is the part of the water potential of a tissue that results from the presence of solute particles. Even though the stomates open to release water‚ it also brings in carbon dioxide to produce sugar and oxygen through a process of photosynthesis
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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Using dialysis tubing to model diffusion of a cell membrane and investigating the influence of solute concentration on osmosis Purpose * Discover how dialysis tubing diffuses the cell membrane * Explore the effect of solute concentration on osmosis Background A cell membrane is a very significant function in the human body. In one sense‚ it is used as a barrier to keep the enzymes‚ DNA‚ and metabolic pathways that bundles everything together. Cell membranes are also used as a gateway
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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reproductive. Orientation is when the organism is placed in their beneficial environment consisting of two behaviors‚ taxis (movement directly towards or away something) and kinesis (random movement). Reproductive is detected using drosophila in this lab‚ it consists of finding courting and mating with another of the species Agnostic behavior is found in a situation where the animal feels threated by another‚ leading to the organism looking bigger or more threatening to the opponent. Within the Agnostic
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Diffusion is a way nonvascular plants can get water. It is the movement of molecules from an area of higher concentration to an area of lower concentration until they are equal. An example of diffusion is red food coloring. When you put food coloring in a glass of water the color will spread through the water until all of it is a red tint. This shows the molecules of the food coloring equally distributed in the area of the water. Osmosis is a special type of diffusion where only the water molecules
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