Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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the effect of temperature on the enzyme amylase was performed to determine the relationship between the enzyme amylase and temperature. The rate of reaction was found to increase as the temperature of the environment was raised. As the temperature was raised from 5°C‚ 20°C‚ 35°C and finally to 80°C the rate of reaction followed this trend and also increased. However as predicted in the hypothesis of this experiment when the temperature was raised too high the enzyme would denature. In this experiment
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McJervis S. Villaruel Professor – BIOL2015(Lab) ABSTRACT This report entitled “Enzymatic activity of Human saliva (Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar plates were prepared and each were labeled 1‚ 2‚ 3‚ 4 and 5 respectively. One mL of saliva were placed in each starch-agar plate which was holed
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amylolytic enzyme found in saliva released from salivary glands. All the enzymes show optimum enzymatic activity around 37° C which is our body temperature. Any deviation from optimum temperature can influence configuration of enzymes which involves folding of polypeptide for acquiring & maintaining its catalytic & allosteric sites. Decline in temperature modifies the catalytic site & the enzymatic activity is significantly lowered & enzyme is deactivated. However‚ deactivated enzyme can resume its
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Interactions BigIdea 4 investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? ■■BACKGROUND Enzymes speed up chemical reactions by lowering activation energy (that is‚ the energy needed for a reaction to begin). In every chemical reaction‚ the starting materials (the substrate(s) in the case of enzymes) can take many different paths to forming products. For each path‚ there is an intermediate or transitional product between reactants and
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Enzyme Report Case 1 - Hereditary Fructose Intolerance 1 & 2. Enzymes take on a variety of roles in the human body at the cellular level. Specifically‚ they aid in the breakdown of macronutrients such as glucose and fructose so that the body can use them. Although reactions within the body would occur without enzymes‚ enzymes provide control because the reaction is not needed all of the time. Enzymes function in specific environments such as temperature or pH; some enzymes are specific
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The cycle starts when the enzymes strip off the CoA portion from acetyl-CoA and combine two-carbon acetyl group with a four- carbon oxaloacetate to form six-carbon citric acid (hence the other name for the cycle is citric acid cycle). The process of creating citric acid is facilitated by enzyme called citrate synthase. Krebs cycle in its simple form is the process of modifying citric acid in several steps
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Conclusion This lab was helpful in showing us how enzyme catalysis really happens in biology. These series of experiments have proven what the optimal conditions and which are not conducive to the functioning of Peroxidase. Table 7.1 was the trial experiment and was performed to observe the overall activity of the enzyme Peroxidase. Each of the three tube mixtures were filled with different concentrations of Peroxidase. As seen on the chart the mixture of tube six and seven had the fastest
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Unit 2: Cell Biolo SCIE206-1401B-08 Photosynthesis and cellular respiration make one large cycle that sustains life on earth. Through photosynthesis the suns energy is constantly being transformed into glucose‚ which is a chemical energy. Respiration is the chemical activity that occurs in all plants and animal cells that release the energy from the glucose. Photosynthesis cannot exist with cellular respiration‚ they are completely linked together in providing the energy through
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Neville Period 1 10/14/12 Enzyme lab Enzymes are biological catalysts that speed up the process of chemical reactions. They are also proteins‚ and most enzymes activities occur within organism. They decrease activation energy‚ energy that is needed to start a chemical reaction. Enzymes are substrate specific substrates ending in "-ase"‚ enzymes ending in "-ase". External factors‚ such as temperature‚ pH‚ and concentration of the substrate‚ affect the enzymes activity in the lab‚ pectase and
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