price‚ and high degree of reduction have made glycerol a highly attractive and exploited carbon source for the production of fuels and reduced chemicals. Here we report the quantitative analysis of the fermentative metabolism of glycerol in Escherichia coli through the use of kinetic modeling and metabolic control analysis (MCA) to gain a better understanding of glycerol fermentation and identify key targets for genetic manipulation that could enhance product synthesis. The kinetics of glycerol fermentation
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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of Shiga toxin producing Escherichia coli O104: H4 outbreak‚ Germany 2011 The Escherichia coli O104: H4 outbreak of 2011 originated in northern Germany and lasted from May through to June of the same year. “The outbreak was characterized by several unusual features: a high incidence in adults (especially women)‚ a greatly increased incidence of the haemolytic–Uraemic syndrome (in approximately 25% of patients‚ as compared with 1 to 15% in previous outbreaks of Shiga-toxin–producing E.coli)‚ a predominance
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HAT 1 Task 3 A. Analyze the selected communicable disease outbreak by doing the following: Describe the communicable disease outbreak. Avian Influenza‚ also known as the Bird Flu‚ is an influenza A virus caused by avian‚ which means bird. These viruses occur naturally in some birds. Wild birds in all areas of the world get the viral infections in their intestines‚ but do not usually become ill from them. The avian influenza is quite contagious among birds and can even kill domesticated
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Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
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Write the heading segment. Specify who the memo is for and who sent it. The heading segment should also include the complete and exact date the memo was written‚ and the subject matter (what the memo is about). A sample heading would look like: To: Name and job title of the recipient From: Your name and job title Date: Complete date when the memo was written Subject: (or RE:) What the memo is about (highlighted in some way) Write the heading segment Always address readers by their correct
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Angelica Beatrice Y. Chong Prof. Mylene Andaya BS Biology Outbreak (1995) Outbreak is movie about a certain virus called Motaba that is highly contagious. Motaba is derived from the place where it originally came from‚ in Motaba River Valley‚ Zaire. From being a deadly virus that is not air-born it somewhat
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The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
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Environmental and Global Health Issues Measles Outbreak and Indicators In the year 2000 measles was considered eliminated in the United States‚ which means it is no longer a prevalent disease in this country. According the Centers for Disease Control and Prevention (CDC) approximately 60 people each year are diagnosed with the measles. However‚ this year‚ in 2013 there were 159 cases reported from January to August. ” This is the second largest number of cases in the U.S. since measles was eliminated
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