3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
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operations in April 1992. Soligen is a metal casting industry which produces fully functional 3-Dimensional cast metal components. Typically the lengths of time to make castings are approximately 6-12month. However with Soligen’s unique technology known as DSPC (Direct Shell Production Casting) the length of production is reduced to weeks or even days. One of the main advantages that Soligen has was the founder Uziel had vast knowledge about metal casting before working with the 3DP process. He was
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Brooks that lots should be cast on who to be put to death to save the rest‚ but Brooks refused to consent‚ and it was not put to Parker‚ that there was no drawing of lots. Why was the idea that casting of lots not be proposed to Parker since I assumed he would be among the lot casted if there was indeed a casting of lots. Third‚ on the same day‚ Dudley proposed again that it would be better to kill Parker and save the others should there be no vessel sighted the following morning. It was Parker’s life
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challenges I faced‚ after we installed electrical pipes inside the concrete slab‚ we found some of them are closed or damaged during casting by casting workers. Meetings were held with the RE manager‚ project manager and the contractor to solve this problem in a minimum financial cost‚ I suggest that we have to redesign all pipes routing through slabs which closed in casting using other pipes which are working. I used my experience in AutoCAD and design to redesign pipes routing and used available pipes
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An Open Access Journal published by ICRISAT ________________________________________________________________________________________________________ Vermicomposting: Recycling Wastes into Valuable Organic Fertilizer Nagavallemma KP‚ Wani SP‚ Stephane Lacroix‚ Padmaja VV‚ Vineela C‚ Babu Rao M and Sahrawat KL. 2004. Vermicomposting: Recycling wastes into valuable organic fertilizer. Global Theme on Agrecosystems Report no. 8. Patancheru 502 324‚ Andhra Pradesh‚ India: International Crops Research
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8/30/13 Venus of Galgenberg – no facial features‚ appears to be dancing‚ vulva is indicated due to the crease between her legs which means it’s female. No idea if used in ritual. Venus of Willendorf – large breasts‚ maybe pregnant‚ covered face‚ clear vulva Venus of Laussel – fingers are clear‚ etchings into horn (indicates wicca)‚ found in a cave entrance‚ clearly holding belly *cornicopias indicate fertility today Venus of Gagarino (looks like Willendorf) - This figure was found in
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1. Compare the characteristics of vending machines and convenience stores as channels A: The main sales channels for soft drinks in japan is Vending machines and convenience store. Vending machines: (1) Japan is known as the country in which vending machines widely spread and it accounts for approximately 40% of sale channels for soft drinks. (2) Each vending machine must be stocked with good balance of product to attract diverse need (3) Secure a prime installation location is difficult unless
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the rod. 3. Learn the correct casting methods. Read on to find out what to look for in these 3 essentials. 1. A Good Rod What makes a good fly fishing rod? Well‚ contrary to some peoples popular beliefs‚ bigger (longer) is not necessarily better. Don’t get me wrong. Length
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the traditional heave-ho method was used for the Great Pyramid. This consists of the workers cutting the limestone out of the quarry and hauling up to the pyramid and setting it in place. Finally‚ in viewpoint #3 ‚ Clifford Wilson claims that the casting method was used. The Egyptians would create wooden moulds which would be set in place for when the liquid cement was poured in and left to dry. This would explain how all of the blocks are approximately the same size and have fairly straight edges
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with uniform physical characteristics. It is a byproduct of ferrous and nonferrous metal casting industries‚ where sand has been used for centuries as a molding material because of its thermal conductivity. It is a byproduct from the production of both ferrous and nonferrous metal castings. The physical and chemical characteristics of foundry sand will depend in great part on the type of casting process and the industry sector from which it originates. In modern foundry practice‚ sand
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