Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23‚ 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology
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Purpose The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity. This lab shows how the enzyme decomposes in hydrogen peroxide. Methods and Materials Refer to handout attached to the back of lab Observations Table 1: The mL of oxygen produced with increase of catalase 30secs 60secs 90secs 120secs 150secs 180secs Disks: 2 17ml 16ml 21ml 26ml 31ml 35ml Disks: 4 8ml 19ml 27ml 35ml 44ml 53ml
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Effects of Enzyme Catalysis of H2O2 by Catalase Report by: Timmy Lin (#269164729) October 17‚ 2011 Mr. Rienzi AP Biology Problem: Measuring the effects of Catalase enzymes on hydrogen peroxide decomposition. Measuring the rate of the reaction when hydrogen peroxide and Catalase are mixed at the same ratio for different time (10‚ 20 30 60 120 180 360 seconds). Background: Enzymes are biological catalysts that carry out cellular metabolic processes with the ability to enhance the rate
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rate of enzymes reactions. One of them is pH‚ which influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment. RESEARCH QUESTIONWhat is the influence of different values of pH on the activity of catalase in potato tuber cells?VARIABLESIndependent: potato discs treatment (placing them into buffer solutions of different pH values‚ mixed with hydrogen peroxide)Dependent: the activity of the catalase‚ reflected
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This section analyzes and presents the results of the two trials conducted‚ including the trial in which Hydrogen peroxide(H2O2) was denatured by a cold catalase solution and the other in which the Hydrogen peroxide(H2O2) was denatured by a hot catalase solution. The experiment conducted displayed signs of experimental error as there were many situations in which the materials provided could have faulted. Theoretically speaking‚ from the inconsistent values provided for the pH levels‚ a possible
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potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds‚ and finally placed at the bottom of the first beaker‚ which contained hydrogen peroxide solution. The timer was then started. The timer stopped when the paper disk floated up to the top of the beaker. This was performed 3 times. After this‚ two negative controls were tested. The first negative control used a denatured catalase instead of the potato extract. The
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Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10‚ 22‚ 30‚ 37‚ 45‚ and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber stopper
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The Effect of Increasing Temperatures on the Enzyme Catalase Enzymes are proteins that help speed up chemical reactions inside your body and without enzymes‚ chemicals reactions in cells would be incredibly slow to a point where no activity at all takes place (Brawo press Inc‚ 2017). Enzymes speed up the chemical reactions by lowering the activations energy and they do this by binding substrates together in the correct orientation to react (Ernest Z‚ 2014). They are vital for life and serve a
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Enzymes are catalytic proteins that speed up chemical reactions by lowering the activation energy of the reaction. our body cells depend highly on enzymes to complete many important functions‚ such as digestion and metabolism. Enzymes are selective‚ in which each enzyme can only speed up a specific reaction. There are molecules that enzymes work with called substrates. substrate bind to an area of an enzyme called active site. There is specificity between the enzyme and substrate that react with
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Enzyme lab report. Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize‚ bind‚ and change specific reactants. They do not change thus can catalyze the same reaction again and again. Activation energy also known as an energy barrier is the amount of energy needed in order to begin a chemical reaction. Catecholase catalyzes the reaction rate of catechol oxidation. Catechol is found beneath the skin of many plants such
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