General Biology I Spring Semester 2013 Lab Report Name: Date: 03/20/2013 Lab: # Purpose of this lab: To test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst‚ which is to say‚ it speeds up the reaction. However‚ extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme
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Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10‚ 22‚ 30‚ 37‚ 45‚ and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber stopper
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varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis HA as substrate concentration increases‚ so will reaction rate until all active sites are bound. In experiment two (i.e. The effect of temperature on the rate of enzyme-catalase reaction) we tested the hypothesis HA as temperature increases‚ so will
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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Bernard Stepteau Biology Lab 101/Th 8:00am – 9:50am 2-26-2014 Dr Laynes Hypothesis: As the temperature of the enzyme catalase rises the activity of the reaction will decrease. Objectives: The objective is to compare catalase activity at different temperatures. Introduction Catalase speeds the breakdown of hydrogen peroxide. Catalase is present in every plant and animal organ. This is useful because hydrogen peroxide is harmful to cells. Temperature effects the enzyme effectiveness
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[pic] P R E F A C E The good name and fame associated with business graduates are attributed to the food high level of studies‚ both inside the classroom and in practical field environment. This aspect has made them prominent among other students. The students of business administration are not restricted to the bookish neither teaching nor they are required to become cramming parrots. They are made to observe how theory is transformed into practical
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influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment. RESEARCH QUESTIONWhat is the influence of different values of pH on the activity of catalase in potato tuber cells?VARIABLESIndependent: potato discs treatment (placing them into buffer solutions of different pH values‚ mixed with hydrogen peroxide)Dependent: the activity of the catalase‚ reflected by the rate at which oxygen is evolved‚ measured at every
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Gold is a soft metal that is largely unreactive. Gold jewellery can lie underground for thousands of years and emerge shiny after that lengthy exposure to air‚ water‚ and other chemicals in the environment. Gold does dissolve in some solvents as indicated below and does react with halogens. The most common oxidation states of gold are Au(I) and Au(III) but there are a few examples of Au(V) (various anions [AuF6]- and [Au2F10]). The Au(III) ion has the d8configuration which gives rise to many square
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Mass of crucible Mass with cuSO4 Mass after heating Difference in mass 29.50 34.50 32.66 1.84 30.40 35.40 33.56 1.84 26.37 31.37 29.54 1.83 29.50 34.50 32.66 1.84 Moles of water = mass/mr = 1.84/18 =0.102 CuSo4 moles = mass/mr = (5 - 1.84)
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Title: The affects of hydrogen peroxide on catalase reactions in animal and plant cells at different temperatures and states. Introduction: All living organisms in the kingdoms of life are composed of and depend on cells to function normally. Not all cells‚ however‚ are alike. There are two primary types of cells: eukaryotic and prokaryotic cells. Cells contain organelles‚ or tiny cellular structures‚ that carry out specific functions necessary for normal cellular operation. (Regina Bailey Updated
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