Computer Enzyme Action: Testing Catalase Activity 6A H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great
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To determine the effects of pH on catalase enzyme activity.Enzyme catalase Lab Statement of the problem : Catalase breaks down H2O2 (hydrogen peroxide) into water and oxygen. H2O2 is a toxic substance formed during metabolism. Hypothesis: To determine the effects of pH on catalase enzyme activity and as you increase the pH the more oxygen that will be produced. Materials: 1. 5 clean test tubes 2. HCI (acid) 3. Dilute HCI 4. Water 5. NaOH (base) Procedure: We start to fill
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designed to investigate the effects of temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which foamed showing a visual
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TEKNOLOGI MARA BIO 411 CELL BIOLOGY PRACTICE 3 REDOX -REACTION/CATALASE ACTIVITY GROUP MEMBERS: Syazani Zahier Bin Zakaria (2014292204) Nur Fatin Syamimi Binti Ahmad Tarmize (2014236664) Zuliakha Binti Zolkafali(2014279698) Nur Izzati Binti Azizan(2014284578) GROUP:ASB1Cb DATE OF SUBMISSION :12 November 2014 INSTRUCTOR: Miss Ellia Kartini Mujar OBJECTIVES 1. To demonstrate reduction-oxidation reaction on living tissue. 2. To compare enzyme catalase on plant and animal tissues. 3. To measure the rate on
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dairy industry‚ the enzyme Protease is used widely. While making cheese‚ protease helps coagulate milk in cheese production. This helps in turning the milk from a soluble product to an insoluble product. Besides‚ the enzyme catalase is used in the medical industry. Catalase helps break down hydrogen peroxide to oxygen and water. This enzyme is used for dressing up wounds. After its catabolism‚ oxygen will heal up wounds faster. In short‚ enzymes help us humans not only inside our bodies‚ but
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Practical: The Effect of the Enzyme Catalase from Chicken Livers on Hydrogen Peroxide [pic] Strand1: SA1‚ SA2‚ SA3 Date: Group Members: Marks: /12 (Part A) + /18 (Part B) = /30 INTRODUCTION: |There are many different reactions that are happening in our bodies all the time. There are reactions that build up molecules‚ | |synthesis/anabolic reactions‚ as well as reactions that break down molecules‚ catabolic reactions. Together
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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After 4 gram stains‚ the slide was blot dried and observed under microscope at 100x with oil immersion. The slide either appear in pink (gram-negative) or purple (gram-positive). Catalase Test: Catalase test enzyme detection of microorganisms‚ catalase hydrolysis hydrogen peroxide (H2O2) into H2O and O2. The catalase test was performed by used a sterile transfer loop been flamed to remove small amount of bacteria from slant agar and placed on the slide. Then
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transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled with catalase in a boiling water bath for five minutes. While we were waiting‚ we rinsed the unlabeled cup we
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