Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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A Quantitative Enzyme Study: CATALASE FlowChart Purpose: Measure the rate of enzyme activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Enzyme Activity Lab Objective: To determine the effect of temperature‚ PH and enzyme concentration on catalase activity. Introduction: What is an enzyme and how the enzyme works. Any of a group of complex proteins or conjugated proteins thatare produced by living cells and act as catalysts in specific biochemical reactions. The system in which enzymes work is often referred to as the ’lock and key’ system it’s where the enzyme and the substrate lock together. The substrate then unlocks into
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Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in
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Biology 20 IB Design Lab Does increasing or decreasing the ph level of a 3% concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules
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Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
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determine how different abiotic conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide‚ water‚ and catalase. The independent variables of the experiment were the abiotic factors such as PH level‚ temperature‚ and the amount
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temperature‚ pH and concentration. Catalase is one of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However‚ when catalase is used it breaks it down into hydrogen and oxygen‚ which is much safer to have in the body. A substrate is the material that is being broken down. In this case‚ the substrate would be hydrogen peroxide. The catalase‚ located in the liver‚ is being
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Taylor Hill Lab 6: Enzymes Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Hypothesis: I believe that the room temperature activity will produce the most activity. Materials: 3% hydrogen peroxide‚ a household blender‚ potatoes (3 medium size potatoes‚ that are peeled and quartered)‚ tap water‚ 8 test tubes‚ 8 transfer pipets‚ sugar packets‚ salt‚ a small saucepan‚ a refrigerator with a freezer‚ stove
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