Introduction: Catalase is an enzyme‚ which is present in all life forms that utilize oxygen for their biological processes. Enzymes are proteins which increase the rate of reaction by decreasing the activation energy (the energy required to initiate a reaction). One of the main function of Catalase is to prevent the accumulation of hydrogen peroxide (H2O2) in the body by breaking it down into water and oxygen gas. Hydrogen peroxide is a toxic by-product of metabolic reactions. If hydrogen peroxide
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In this experiment we are trying to determine the effect temperature has on catalase activity. We started out with five test tubes and then labeled them as we went about‚ after we added a catalase extract to each tube. Once added to each test tube they all sat in their respected temperature for ten minutes‚ so they could have a full effect. Once the time was up each was then collected and then we began to shake them from side to side for the foaming to start. When the foam settled‚ we discovered
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Catalase is a common enzyme found in the bodies of all living organisms in contact with oxygen. It is involved in reacting with hydrogen peroxide to convert it into water and oxygen and can do so with millions of hydrogen peroxide particles each second. These types of enzymes have many uses in bodily systems commonly known as proteins that speed up the rate of the metabolic process by regulating the chemical process which helps digest food and break down toxic substances. The most favourable conditions
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the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar
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independent variable in this investigation is the varying concentrations of the substrate (Hydrogen Peroxide: 1%‚ 3%‚ 5% and 6%)The dependent variable was the rate of enzyme catalase activity‚ which was measured by the volume of froth produced after one minute.The concentration and volume of liver extract (source of catalase) used was kept constant at 10ml per solution. This factor was kept constant‚ as the concentration of the enzyme affects the rate of reaction; higher enzyme concentrations increase
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EFFECT OF TEMPERATURE ON THE RATE OF CATALASE REACTION IN HYDROGEN PEROXIDE INTRODUCTION Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer‚ 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products‚ or where two substrates join up to form one product (Dundon‚ 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal
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The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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experiment was the enzyme‚ catalase. An enzyme is a specialized protein which catalyzes reactions in cells. The enzyme in our experiment was catalase‚ which came from the potato juice. The function of an enzyme is to catalyze reactions in cells. The specific function of the enzyme in this experiment was to see if it affected the activity if it was exposed to different temperatures. A substrate is a material or substance which the enzyme acts. The substrate in our lab was hydrogen peroxide.
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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