bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table 1). After starch hydrolysis‚ I ran an MSA plate‚ hoping it would give
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In this lab‚ I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than
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After 4 gram stains‚ the slide was blot dried and observed under microscope at 100x with oil immersion. The slide either appear in pink (gram-negative) or purple (gram-positive). Catalase Test: Catalase test enzyme detection of microorganisms‚ catalase hydrolysis hydrogen peroxide (H2O2) into H2O and O2. The catalase test was performed by used a sterile transfer loop been flamed to remove small amount of bacteria from slant agar and placed on the slide. Then
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Catalase Enzyme Lab Samiya Hussein March 9‚ 2012 Introduction In order to receive the necessary amounts of energy required for daily function‚ the digestive system must break down proteins‚ fats and carbohydrates. In doing so‚ the body produces poisonous chemicals; however‚ the cells aren’t harmed. This is because enzymes are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide
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(SBI 4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in the form of heat which
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decreasing the energy of activation. The main function of enzyme catalase is to convert hydrogen peroxide (H2O2) in our bodies into oxygen and water. This can be visually seen when hydrogen peroxide is put on a wound and the peroxide bubbles. Enzymes can also be found in plant cells and fungi. (Huston.) In this experiment we test the many variables that can change the rate of this reaction such as temperature‚ concentration levels of enzyme catalase and pH values. We are able to track these changes using
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Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4‚ there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However‚ oxygen can be
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INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase’s different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a
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4.3 (b) To investigate the effect of pH on the rate of catalase activity Teacher Notes Apparatus required per class group of 24 students Chopping board 12 Knife 12 Weigh boats 12 100 ml Graduated cylinders 36 Boiling tube 36 Syringe 12 Electronic balance 3 Dropper 12 Timer 12 Labels 36 Thermometer 12 Water bath 3 Enzyme source eg. Radishes/celery Hydrogen peroxide Range of buffer solutions pH paper Washing up liquid Disposable gloves The apparatus
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CATALASE Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in reproductive reactions. Likewise‚ catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of molecules of hydrogen peroxide to water and oxygen each second. Catalase is a tetramer of four polypeptide chains‚ each over 500 amino acids long. It contains
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