EXTRACTING AND PURIFYING GENOMIC DNA FROM A RAT LIVER FOR ELECTROPHORESIS Deoxyribonucleic acid (DNA) is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses. Along with RNA and proteins‚ DNA is one of the three major macromolecules essential for all known forms of life. Genomic DNA is the DNA that holds the complete set of genetic data for an organism. In humans‚ the genomic DNA spans 46 chromosomes‚ providing a complete
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Taylor Durham Nutrition 101 Mr. Oropallo Lab Report #2: Fat Extraction From Food What is a triglyceride? Draw its structure. A molecule that contains three fatty acids attached at the oxygen atoms of glycerol. What are the differences between saturated and unsaturated fats? Drawn an example of each. If all bonds are single‚ the fatty acid molecule is saturated. If there is a double bond among the carbon atoms‚ the fatty acid molecule is unsaturated. What form does each of these fats take at
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Abstract: An enzyme is a protein that serves as a biological catalyst (Denniston‚ 2007). Metabolic reactions happen with in cells. Enzymes are proteins that are used to speed up these reactions without being consumed by them (Mader‚ 2010). Catalase is a catalyst that digests potent hydrogen peroxide and converts it into H2O and O (Campbell Reese‚ 2008). The environment plays an important role in the reaction that enzymes have. In this experiment‚ enzymes were exposed to changes in temperature
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Krupa Desai Cell Biology April 3‚ 2013 Lab: Biosynthesis of Starch Introduction: In this lab we learned the concept and procedure of synthesizing starch. We also learned the effects of pH and temperature on the reaction rates of amylase.. In the process of the synthesis lab we learned phosphorylation using a potato‚ which was what we synthesized. The phosphorylation took place after the addition of primer. There are two different types of starches used are amylose and amylopectin. To test
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Enzyme Lab Report Introduction: Enzymes are proteins that enable chemical reactions. In the enzyme lab‚ the effects of concentration‚ temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction. Materials and Methods: Experiment 1‚ 2‚ & 3 Experiment 1 examined the effects of concentration on catalase activity. Experiment 2 examined the effects of concentration in temperature
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bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or cream-colored‚ with irregular borders.} Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain
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evolved (gas produced)‚ the more reactions were being produced/higher rate of reaction. Different types of enzymes’ reaction proportions differ based on the pH being added. For example‚ intestinal enzymes may react differently to pH than liver enzymes (catalase) which was tested. The hypothesis was proved to be correct due the rate of reaction increasing as the pH levels got more basic. Based on Graph 1‚ the slope of the pH 9 line was more
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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Moment of Inertia and Rotational Motion Garret Hebert PHY 2311 Tues 1:00 garret.hebert@hindscc.edu Abstract: During this lab we will study what rotational Inertia is and how different shapes of masses and different masses behave inertially when compared to each other. We will specifically study the differences of inertia between a disk and a ring. We will use increasing forces to induce angular acceleration of both a disk and a ring of a certain mass. We will then then measure the differences
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Gonzales ID#: 810004692 Lab Partner: Onecia Alexander Date: Friday 23rd October 2015 Theory: Arginase is an enzyme with the E.C. number 3.5.3.1 (Worthington 2015). This details it as a hydrolase enzyme that catalyses the cleavage of bonds through the addition of water. For this experiment‚ we pay close attention to the reaction where arginase catalyses the hydrolysis of arginine to ornithine and urea. This reaction is a part of the urea cycle and occurs in mammalian livers and sometimes kidneys
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