Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10‚ 22‚ 30‚ 37‚ 45‚ and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber stopper
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because the high amount of salt causes more dehydration. Variables: Independent- Salt Solution Dependent- Mass of Potato Control- Tap Water Materials: •Tap Water (50 ml for each cup) •Salt •Beaker •3 cups •3 pieces of potato •Scale Procedure: Step 1: Gather Materials. Step 2: Weigh each potato separately on the scale and record weight in grams on table. Step 3: Put each potato in a separate cup and add 50 ml of water to each cup‚ measure with beaker. Step 4: To create the 40% salt solution
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Lab Report Purpose: Osmosis and diffusion through potato core in different concentration (viable) : The concentration of solution in a solution will affect the movement of water across potato cell membrane Material: Potato core Sucrose solution (0.2‚0.4‚0.6‚0.8‚1.0) Electronic Balane Test tube and rack Scalpel Plastic Weighing tray Cork Borers Method: Day 1 use you cork borers to bore 6 vertical hole on your potato. remove the potato cylinders from
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Biology investigation: Liver and hydrogen peroxide By Cuong Tran Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced‚ while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found
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An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase‚ during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic
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If the pH level of the solution is more basic‚ then the potato membrane will weigh less due to osmosis. Our null hypothesis was: If the pH level of a solution is either acidic or basic‚ then it will have no effect on the weight. The independent variable was the different solutions of different pH values. The dependent variable was the weight of the potato‚ which was measured in grams. The baseline of this experiment is the weight of the potato when in the milk solution because milk has a neutral pH
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Discussion: The liver homogenate was expected to have the highest amount of protein concentration‚ DNA concentration‚ and total glucose when compared to the other homogenates. The liver was the biggest organ in B. taurus and it carried out numerous functions such as producing bile‚ synthesizing proteins for blood clots‚ and recycling old red blood cells. The liver ended up containing the most DNA concentration‚ but it was surpassed in protein concentration by the kidney. The protein concentration
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from the experiment demonstrates that the catalase enzyme breaks down the hydrogen peroxide due to its harmful toxicity to the liver. In section A‚ the effect surface area has on the enzyme was tested. The results have proven that as the surface area increases‚ the reaction rate of the enzyme also increases. To illustrate‚ when the liver was ground‚ the bubbles from the reaction reached a maximum height of 150mm in five seconds less than the unground liver which merely reached a maximum height of
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varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis HA as substrate concentration increases‚ so will reaction rate until all active sites are bound. In experiment two (i.e. The effect of temperature on the rate of enzyme-catalase reaction) we tested the hypothesis HA as temperature increases‚ so will
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Effect of Temperature on Enzyme Activity Bernard Stepteau Biology Lab 101/Th 8:00am – 9:50am 2-26-2014 Dr Laynes Hypothesis: As the temperature of the enzyme catalase rises the activity of the reaction will decrease. Objectives: The objective is to compare catalase activity at different temperatures. Introduction Catalase speeds the breakdown of hydrogen peroxide. Catalase is present in every plant and animal organ. This is useful because hydrogen peroxide is harmful
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