Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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Broadly speaking‚ enzymes are proteins that is produced to perform as a biological catalyst in chemical reactions. Catalyst are used to increase the rate of a chemical reaction. In this study‚ we performed two different experiments that investigated the effect of varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis
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Enzymes What Are Enzymes? Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes. Enzymes are specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate
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Enzymes are catalytic proteins that selectively speed up chemical reactions without by consumed by the reaction itself (1). Enzyme activity is significantly affected by factors such as temperature‚ substrate concentration‚ enzyme concentration and pH. Enzymes are highly specific and only catalyse one specific chemical reaction‚ speeding up the reaction by lowering the activation energy‚ the energy required to start a chemical reaction (2). At high temperatures and with various mechanical forces or
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(Justification-2 Enzyme Inhibition) By quantitative balance‚ the total amount of Enzyme is [E] 0= [E] + [EI] + [ES] + [ESI]. By using a=1+[I]/KI and a′=1+[I]/K′I‚ it is followed by [E]0=[E]a+[ES]a′ This equation can be written like this‚ [E]0=(Km[ES])/([S]0)a + [ES]a′=[ES]( aKm/[S}0+a’)‚ because of Km=[E][S]/[ES] and [S]≈[S]0. V=kb [ES] =kb [E] 0/ (aKm/[s] 0+a’). Kb [E] 0 is Vmax. This is why V=Vmax/(a^’+aKm/[S]0). This equation can be rearranged like this‚ 1/V= a’/Vmax+(aKm/Vmax)1/[S]0‚ which is
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Catalysts are compounds that accelerate the progress of a reaction without being consumed. They have the ability to increase the rate of reaction in a chemical reaction. Potentially‚ molecules that would once have taken years to interact‚ can take seconds with the addition of a catalyst. The overall purpose of a catalyst is to ensure that reactions proceed effectively which is why a range of catalysts are commonly used in many elements of society. Common examples of where catalysts are used include;
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Enzyme action is the simple mechanism by which enzymes catalyse chemical reactions. This begins with the binding of the substrate to the active site on the enzyme. The binding of the substrate to the enzyme causes changes in the distribution of electrons in the chemical bonds of the substrate. This then causes the reactions that lead to the formation of products that are then released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique shape that
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EFFECT OF TEMPERATURE ON THE RATE OF CATALASE REACTION IN HYDROGEN PEROXIDE INTRODUCTION Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer‚ 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products‚ or where two substrates join up to form one product (Dundon‚ 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal
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Name: ________________________________ Date: __________________________ Lab Partner: _____________________________________________________________________ Investigating Factors that Affect the Rate of Enzyme Activity See Textbook Pg 61. Enzymes are very large complex organic molecules that are synthesized by the cell to perform specific functions. These biological catalysts are important because they speed up the rate of the reaction that would otherwise be too
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