pubs.acs.org/joc Mechanism and Regioselectivity of the Osmium-Catalyzed Aminohydroxylation of Olefins Dominik Munz and Thomas Strassner* Physikalische Organische Chemie‚ Technische Universit€t Dresden‚ Mommsenstrasse 13‚ 01062 Dresden‚ a Germany thomas.strassner@chemie.tu-dresden.de Received November 12‚ 2009 The mechanism and regioselectivity of the osmium-catalyzed aminohydroxylation of olefins was investigated in detail by density functional theory (B3LYP/6-31G(d)) calculations in
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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The experimental results were different from the hypothesis because while the enzyme appeared to not work as well‚ I expected a more significant change. Most of the time‚ there was only a millimeter of difference of the foam between the two samples while I expected a greater difference such as 10 millimeters. Enzymes speed up chemical reactions and at the active site‚ a substrate can be broken down or two substrates can form a larger molecule. Hydrogen peroxide is broken down by peroxidase into water
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Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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Most people choose hydrogen peroxide over rubbing alcohol to clean a wound since hydrogen peroxide doesn’t burn. However‚ when hydrogen peroxide is treated on deeper wounds into the flesh‚ it can pose numerous problems to cellular health. When hydrogen peroxide is used correctly to clean wounds‚ it becomes an oxidizer and can be used as a disinfectant to prevent infections. However when used incorrectly‚ hydrogen peroxide can cause oxidative stress on cells. This essentially means that there is an
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Amylase is a very important enzyme located in the saliva and pancreatic juices that hydrolyses (break down) starch and glycogen into more simple and readily digestible forms of sugar. Enzymes are biological catalysts that increase the rate of biological reactions. Enzymes are produced in living cells and are involved in speeding up biochemical reactions. They have an active site to which specific substrate binds. They increase the rate of reactions by decreasing the amount of activation energy meaning
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The Effect of Increasing Temperatures on the Enzyme Catalase Enzymes are proteins that help speed up chemical reactions inside your body and without enzymes‚ chemicals reactions in cells would be incredibly slow to a point where no activity at all takes place (Brawo press Inc‚ 2017). Enzymes speed up the chemical reactions by lowering the activations energy and they do this by binding substrates together in the correct orientation to react (Ernest Z‚ 2014). They are vital for life and serve a
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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