Lab Ex#8: "Enzymes: Catalysts of Life" INTRODUCTION Enzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity‚ we would not possess the catalyst activity necessary for energy to produce movement. Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables‚ such as temperature
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Effects of Temperature and Cofactors on Enzymatic Reactions “I pledge that no unauthorized assistance has been given or received in the completion of this work. Experiments described were performed by me and/or my lab group and this write-up is entirely my own creative work.” X________________________________________ Introduction Enzymes are protein molecules that speed up the rate of reactions by reducing the activation energy of a reaction. They act as catalysts in reactions‚ increasing
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Did different conditions affect the Enzyme’s Efficiency? The hypothesis was “the more the imposed condition affects the mobility of the Enzyme‚ the fewer toothpicks will be broken”. This hypothesis proved true. This experiment manifested through multiple trials that the condition the enzyme had to endure definitely affected the Enzyme’s Efficiency. During this modeling experiment‚ it was observed that that the efficiency of the Enzyme over time created a line like that of a radical function
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Enzymes are globular proteins that are synthesized by the ribosomes in a cell. They act as catalysts during biological reactions; therefore‚ enzymes are able to speed up these reactions without undergoing a permanent change themselves. These proteins are able to do this by lowering the activation energy of a reaction. To add on‚ enzymes require specific conditions under which they can work best. Reactions occur at faster rates when the temperature is higher. However‚ the rate of an enzyme-catalyzed
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Abstract: Enzymes are part of human’s everyday lives and improve the quality of living. Enzymes decrease activation energy which pertains to how much energy is needed for chemical reactions to take place. Enzymes also known as catalysts is one of the main factors in producing energy in individual’s bodies. The experiment conducted was to test how different temperatures effected the catabolizing of fungal and bacterial amylase‚ as well as the optimal temperature needed for the enzyme to correlate
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Abstract This experiment investigated the kinetics of the enzyme glycogen phosphorylase b which is important to metabolism. AMP is an allosteric activator of the enzyme because it converts glycogen phosphorylase b from its T state to the R state which is the active form. Caffeine is an inhibitor because it binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen
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In biology‚ energy is needed for any process or reaction. Life would not exist without the presence of enzymes (Phillips‚ 2017). Through chemical reactions‚ this energy is created and is controlled by a catalyst‚ enzymes. Enzymes are known as proteins that are produced in living cells that speed up the metabolic processes of an organism. These catalysts speed up these reactions by decreasing the activation energy‚ how much energy is needed for a chemical reaction to happen (WBC‚ 2015). An enzyme-substrate
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Factors affecting Enzyme Activity: The effect of pH on enzyme activity Background Knowledge: An enzyme is a biological catalyst – which speeds up the reaction rate‚ without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme.
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Enzymes are biological catalysts that speed up chemical reactions by lowering the amount of activation energy needed to start the reaction and let the reaction occur at temperatures found in living cells. The way that enzymes do this is explained with the lock and key hypothesis. This hypothesis says enzymes have a specific shape called the active site which is different between different enzymes. Molecules called the substrate that participates in the reaction also have a specific shape that can
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5. What determines where a protein kinase or protein phosphatase will perform its enzymatic activity? Answer: As protein kinase and protein phosphatase are the enzymes‚ they both perform their enzymatic activity by interacting at the specific binding site on the protein. Both of the enzymes recognize the specific amino acid sequence around the amino acid that is to be either phosphorylated or dephosphorylated respectively. 6. Why would a signaling pathway need to be regulated? Answer: Signaling
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