INTRODUCTION Enzyme is a biological catalyst that acts on a molecule called substrate and it also significantly speed up a chemical reaction by lowering the activation energy. In order to learn about the enzyme and its behaviour‚ this lab practical is conducted to examine the kinetic of the enzyme alkaline phosphatase. As illustration‚ when alkaline phosphatase is added to a substrate called p-Nitrophenyl phosphate (colourless in alkaline solution)‚ a series of reaction takes place and eventually
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Enzymes are a type of protein. They are catalysts meaning they speed up the rate of the reaction. Enzymes activity depends on the concentration of the substrate‚ temperature and the pH. The more concentrated the substrate is the more reactive the enzyme is. The optimal pH for an enzyme is 7.5 and the optimal temperature for an enzyme is 53 Celsius. Extremes in the temperature and the pH of an enzyme can denature therefore destroy it. The enzyme that is in this experiment is Amylase. Amylase is found
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An enzyme is a biological catalyst‚ meaning that it is a substance that possess the ability to increase or speed up the rate of reaction without itself being used up in the process. Enzymes provide an alternate reaction pathway by lowering the activation energy needed for a reaction to occur. For two molecules to react they must collide with each other‚ however‚ they have to collide with sufficient energy. Sufficient energy means that between them they have enough energy to overcome the energy barrier
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Skip to Main content ADVERTISEMENT Journals Books Shopping cart Sign in Help Top of Form Advanced search Bottom of Form Catalysis Today Supports Open Access | About this Journal | Sample Issue Online | Submit your Article New Article Feed Alert me about new articles Add to Favorites Copyright © 2014 Elsevier B.V. All rights reserved < Previous vol/iss | Next vol/iss > Volume 190‚ Issue 1‚ Pages 1-150 (1 August 2012) Alternative Sources
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SBI 4U0: Enzyme Lab Purpose: To compare the action of the enzyme catalase‚ to a non-protein catalyst under different conditions. Observations: | | |Observations |Rate of Reaction |Interpretations | |A |Sand |- Sand piled up at the bottom of |0 |- There is no reaction between sand and| | |
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BIO 211 Lab Section 11 February 15‚ 2012 Effects of Temperature on Enzymatic Activity Abstract Temperature is a measure of kinetic energy. As this movement increases‚ collision rate and intensity‚ and therefore reaction rates‚ increase. This experiment was conducted to determine if there is a minimum temperature that increase kinetic energy and denature enzymes to slow enzymatic reactions or fail to catalyze them. The experimental results indicate an increase in temperature will increase reaction
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Expt. BL 304 Kinetics of Alkaline Phosphatase Objective To study the kinetics of alkaline phosphatase. Procedure 1. Sodium carbonate - sodium bicarbonate buffer: Dissolve 20 ml of 0.2 M solution of sodium carbonate (2.12 gm in 100 ml distilled water) and 230 ml of 0.2 M sodium bicarbonate (4.12 gm in 250 ml distilled water) to make up the volume 250 ml with pH 9 -9.2 2. 5 N sodium hydroxide solution (10 gm in 50 ml distilled water) 3. Substrate stock solution: Dissolve 0.1 gm of p-nitrophynyl
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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Factors Affecting Enzyme Action ------------------------------------------------- Term 1 Biology Nicole Goosen Table of Contents Introduction: 3 Materials: 3 Method: 4 Risk assignment: 4 Risk: 4 Personal Protective Equipment: 4 Results: 5 Discussion: 5 Conclusion: 6 Introduction: How is the human body able to digest the food that you eat? How quickly your body digests your food? This is because the human body contains enzymes that are the biological substance‚ a.k.a proteins
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Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes‚ enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide‚ chicken meat‚ apple‚ carrot‚ potato‚ or chicken liver] I think that the manganese dioxide‚ apple‚ and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
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