Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates Inhibition of enzyme activity pH
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The activity of an Enzyme is affected by its environmental conditions and changing these factors can alter the overall rate of the reaction. Reaction rates are influenced by external factors such as pH‚ temperature and salt concentration. Different enzymes have different optimum temperatures in which they are most efficient and different pH levels which is ideal for their formation of enzyme-substrate complexes. As the temperature increases‚ there is more movement of molecules and more random collisions
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jellies‚ these were made by putting sliced pineapple into the jelly before it set‚ the following observations were made: oThe jelly set normally when tinned pineapple was added. oWhen fresh‚ unripe pineapple was used the jelly set but was not as firm. oWhen fresh‚ ripe pineapple was used‚ the jelly did not set at all But why? The reason the jelly is not setting is due to protease‚ which is breaking down the protein in the jelly‚ protease is an enzyme‚ and its rate of activity is affected by many
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Enzyme lab report. Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize‚ bind‚ and change specific reactants. They do not change thus can catalyze the same reaction again and again. Activation energy also known as an energy barrier is the amount of energy needed in order to begin a chemical reaction. Catecholase catalyzes the reaction rate of catechol oxidation. Catechol is found beneath the skin of many plants such
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Title: The Effect of Varying Amounts of Substrate and Enzyme on a Reaction Rate Abstract In living organisms‚ certain reactions must take place rapidly to assist life. This occurs because of enzymes‚ because all reactions would take place too slowly to sustain life (Jacklet‚ 237). Enzymes are large protein molecules that catalyze specific chemical reactions without being used up in the process. Each enzyme has a region on its surface‚ called the active site‚ which recognizes a specific
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------------------------------------------------- Enzyme Pre-Lab Harry Kang 9/26/12 1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions‚ such as‚ different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with‚ 1.5 ml of H2O2‚ 1.5ml of H2O and different amounts of enzyme drops‚ to determine how much oxygen gas is produced during the reaction
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Company Background The Australian Pineapple Products Exporting Company (APPEC) was incorporated in Central Queensland‚ Australia by a group of 10 top pineapple-growers and merchants (5 large individual growers‚ 1 large fruit merchant already exporting‚ 4 packing sheds representing groups of growers). These 10 pineapple-growers are among the best operators from each of the four main pineapple-growing districts‚ Central Queensland‚ North Queensland‚ Southern Queensland‚ and the Wide Bay area near
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Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet
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Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase‚ thus increasing the rate of reaction. However‚ if the temperature is too high the enzyme will denature. Materials: 4 test tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato
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This experiment was performed in order to analyze the effects of pH‚ enzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic
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