Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol Carson Levine November 4th‚ 2013 Abstract Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is exposed to oxygen. When the reaction occurs‚ benzoquinone is produced turning the oxidized substance brown. It was hypothesized that the higher the concentration of catechol oxidase‚ the browner the substance will turn
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Studying the Rate of Reaction of catechol oxidase and how it affects pH levels Introduction: In this lab‚ we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in
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As mentioned before‚ browning in bananas is an enzymatic process where enzymes such as polyphenol oxidase serve as catalysts in the oxidation of several phenolic compounds. In this experiment however‚ the only reaction being tested is the oxidation of catechol (a diphenol) by the enzyme catalyst catechol oxidase or polyphenol oxidase‚ which initiates a chain of reactions and eventually triggers the formation of brown pigments known as catechol-melanins. Catechol‚ also called 1‚2-dihydroxybenzene
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Why is your study significant‚ i.e.‚ how is your objective relevant/applicable to the larger world and why would the information you seek be important/valuable to others? (either in terms of using catechol oxidase as a model to study enzyme function or considering the specific activity of catechol oxidase) 3) State your hypothesis (an educated prediction): Materials and Methods 1) List specific materials used in your experiments (and sources of these materials). Also list any specialized
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of microorganisms because it is toxic to them (Danyk‚ H‚ 2013). Quinones are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk‚ H‚ 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract and water. To provide more results of the enzymes productivity patterns the solutions; temperatures
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Respectively‚ 1‚ 2‚ 4‚ 8‚ 16‚ and 24 drops of catechol were added to each of the tubes. To equal out the amount of solution in each of the test tubes‚ respectively 23‚ 22‚ 20‚ 16‚ 8 and 0 drops of pH 7 buffer were added. 30 drops of potato juice containing the catechol oxidase enzyme were then added to the solution‚ and then timed for 5 minutes at room temperature. The pH treatment only required 5 test tubes‚
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Lab: Inhibiting the Action of Catechol Oxidase By: Kimberly G. Introduction: In this lab‚ Mr. Greene ’s sixth period AP Biology class split into groups "to investigate inhibition of enzyme activity by specific chemicals called inhibitors" (1). Group three pondered this lab ’s inhibitor‚ phenylthiourea (PTU). Is it a competitive inhibitor? That is an inhibitor that literally "competes" with the substrate by mimicking it‚ and thus "wins" the position at the active site of the enzyme. The blocked
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Glucose Oxidase and Its Various Uses Aaron Truong Since glucose oxidase has an end product called hydrogen peroxide‚ which is a harmful substance to bacteria‚ it can be used to fight bacteria‚ or sterilize objects (can have various uses such as in hand sanitizers‚ toothpaste‚ soap‚ etc)‚ not just biosensors. Another key part in the reaction would be C6H12O6‚ or glucose. Glucose oxidase can be applied to diabetics as mentioned earlier‚ as biosensors work by "keeping track of the
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative.
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extract in phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts of phosphate
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