Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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(min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15 Brown/Red Clear/Milky Clear/Milky How can benzoquinone be detected? Benzoquinone can be detected if you see a brownish amber color in the solution Which two solutions need to be present to produce the brown-colored benzoquinone? Potato extract and catechol need to be present What
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3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5 minute intervals for observation) Materials: 4 test tubes Test tube rack Wax pencil Small ruler Buffer solutions of pH 10‚ 7‚ 6‚ and 3 Potato extract Catechol Procedure: 1. Using the small ruler and the wax pencil‚ three markings are made on
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to the following direction. 4. Continue your work area and add 1cm of potato extract obtaining catechol oxidase to each of the seven tubes. 5. Add 1% catechol to each of the 7 test tubes‚ bringing the total volume to the 6c mark. Agitate the contents of the tubes using a vortex mixer if available. 6. At time 0‚ record the relative color intensity of each tube immediately after adding the 1% catechol. 7. Place the tubes in 40’C water bath. 8. Agitate the tubes periodically over the next ten minutes
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out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop the effects of catechol oxidase on potato cells by testing the change in absorbency over time. Our data supported our hypothesis that EDTA
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at 1cm and 2cm from the bottom. We then identified each test tube as 2a‚ 2b‚ and 2c. - We filled each test tube with the following: 2a with 1cm of potato extract containing Catechol Oxidase and 1cm of 1% Catechol Solution; 2b with 1cm of potato extract containing Catechol Oxidase and 1cm of dH2O; 2c with 1cm of 1% Catechol Solution and 1cm of dH2O. We tapped each tube to completely mix the solution and recorded the time at zero. - At zero time‚ each test tube was scaled for color concentration
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the activation energy (Reece and Campbell). Because enzymes are so specific in their shape‚ they will only work on substrates that fit‚ and no others. In this lab we utilized Catechol Oxidase as our enzyme‚ and Catechol as our substrate. When exposed to oxygen catechol gradually changes to benzoquinone‚ catechol oxidase speeds up this reaction. We tested the effects of temperature‚ pH‚ and inhibitors on this enzyme system. This was all accurately timed and measured to ensure proper results. My
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Effect of Enzyme and Substrate Concentration on Reaction Rate by Zachary A. Poché Biology 155 Laboratory October 15‚ 2014 Lab Partners: Cade White‚ Hannah Ragas‚ Russheka Aremillion ABSTRACT In order to increase the reaction rate‚ substrates attach to the active site of enzymes which decrease the activation energy required to convert substrates to products. We examined the effect of enzyme concentration and substrate concentration on the overall rate of the reaction. To determine
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measuring and recording the different levels of substrate and enzyme we recorded all the data that corresponded to the change after the level of catechol was changed as well as observing the different pH on the same enzyme. Materials List 1. Test tubes 2 .Test tube rack 3. Small Para film squares 4. Distilled water 5. Potato extract 6. Catechol 7. Phenylthiourea 8. Disposable gloves 9. Pipette 5ml 10. Pipette 1ml 11. Bunsen burner 12. Ice 13. Ice chest 14. Thermometer 15. pH solutions
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this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant. Our findings illustrate that the rate of enzyme activity is only influenced by substrate concentration at low level of substrate concentration‚ and as substrate concentration
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