Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close
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benzoquinone production when altering the pH and temperature of the reaction‚ as well as enzyme and substrate concentrations. We used a Spec 20 to evaluate absorbance at 540 nm. Absorbance represents rate of product formation. We tested the substrate catechol and the enzyme catacholase and recorded the product benzoquinone. The objective was to identify the optimal conditions for the enzyme catacholase. I predicted that the reaction rate will rise with increasing enzyme concentrations until an optimal
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Each tube had additional 47‚ 46‚ 44‚ 40‚ 32‚ 24‚ and 16 drops (one tube had no additional drops respectively. Each tube had drops of catechol added with respect to the numbered label (test tube 1 had 1 drop of catechol‚ test tube 16 had 16 drops‚ etc.). After these solutions were mixed‚ each tube was covered with Parafilm and inverted 3-4 times. The film was removed from the tubes and 30 drops of diluted potato juice was added
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Title: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Aim To design and conduct an experiment to demonstrate the presence of enzyme activity in the preparation provided. To examine the effect of the inhibitors provided. To test whether the other phenolic substrates provided can be oxidized by the enzyme preparation. To test for the presence of peroxidase activity in the enzyme preparation. To test the effect of the inhibitor provided on peroxidase activity
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of the enzyme and the rate of the reaction. The browning of potatoes when they are peeled is caused by catecholase‚ an enzyme‚ as it facilitates a reaction between between catechol and oxygen. In this reaction‚ oxygen’s electronegativity separates 2 hydrogen atoms from catechol‚ oxidizing it and therefore converting catechol to benzoquinone molecules that forms long‚branched chains. The chains are the backbone of the red and brown pigments that cause darkening.
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The control group of this experiment was room temperature. The constant variables were‚ the amount of distalled water‚ catechol substrate‚ and potato extract in each tube was the same. (2ml) The size of the test tubes were the same also.
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large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped and mixed by inversion. After mixing‚ 1.0mL of catechol was added to each of the five experimental solutions and
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The purpose of this experiment is to study how enzyme activity is affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in
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recalibrating it. The additional 4 experimental test tubes were composed of the same contents but 1 mL of the substrate catechol was added instead of the 1 mL of water (Table 2). These test tubes were labeled the same as the previous blanks. The spectrophotometer wavelength was set to 420 nm just like exercise A. After the spectrophotometer was adjusted using the blank A‚ the catechol was added to tube A. The tube was covered with paraffin and the absorption was measured within 5 seconds. The absorbance
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water‚ 6mL of Catechol‚ 1.5mL of Extract(potato)‚ a Spectronic 20 (spectrophotometer)‚ Wax pencil‚ a Test tube rack‚ three Parafilm‚ tissues to clean the Spectronic 20 and the test tubes‚ 3 pipets and a timer. So we won’t confuse ourselves‚ we used the wax pencil to label each test tube as we placed the required amount of substance in each of them. To avoid any slight errors‚ we also used three different pipets for the three required materials‚ which are the distilled water‚ catechol and the extract
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