Part 1 dilutions participated as a trail run to gather around the “full enzyme” that was used for the other parts of the experiment. Part 2 readings were in the time span of 10 minutes and the final results show a spontaneous increase in the first minutes of the absorbance readings. They then showed a steady reading towards the end of timing. The amount of enzyme is decreasing as the dilution occurred. The activity it catalyzes measures the amount of enzyme present in the reaction (Introduction to
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The hydrolysis was performed using ACR in a concentration of 5 FPU.g-1. The EH was performed following the standard conditions in sequential and in simultaneous reactions. After the hydrolysis‚ glucose concentration was determined using the glucose oxidase kit (Doles Reagents ®) following the manufacturer’s guidelines. The results were compared by statistical analyses (Student’s t testy-test). Significant differences were obtained when p ≤
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Microbial analysis of soil‚ of top layer from selected sites of Area near Dahisar River Saika N. Esani University of Mumbai (Email – saikae@ymail.com) Abstract: soil samples were collected fortnightly from area near Dahisar River‚ A river in suburb of Mumbai. laboratory analysis started from July 2010 to September 2010. Total bacterial and fungal count were estimated by standard spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their
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Pt nanoparticles onto a highly-oriented titania nanotube array. The prepared electrode‚ characterized by SEM and EDX‚ shows remarkably improved catalytic activities in the oxidation of hydrogen peroxide. By modifying the electrode with glucose oxidase (GOx) the resultant glucose biosensor exhibits a high sensitivity to glucose in the range of 0 to 1.8 mM with a response time of 3 s and detection limit of 0.1 mM. © 2008 Elsevier B.V. All rights reserved. 1. Introduction Electrochemical biosensors
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Carbohydrates General molecular formula Cn(H2O)n Appeared to be hydrates of carbon. not all carbohydrates have this empirical formula: deoxysugars‚ aminosugars Carbohydrate - polyhydroxy aldehyde‚ ketones. General characteristics Most carbohydrates are found naturally in bound form rather than as simple sugars Polysaccharides (starch‚ cellulose‚ inulin‚ gums) Glycoproteins and proteoglycans (hormones‚ blood group substances‚ antibodies) Glycolipids
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May 1‚ 2013 Enzymes as Drug Targets Enzymes are defined as any of numerous proteins produced in living cells that accelerate or catalyze the metabolic processes of an organism. Enzymes are usually very selective in the molecules that they act upon‚ called substrates‚ often reacting with only a single substrate. The substrate binds to the enzyme at a location called the active site just before the reaction catalyzed by the enzyme takes place. Enzymes can speed up chemical reactions by up to
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1. Phenelzine (Nardil) - antidepressant‚ monoamine oxidase inhibitor. -Indicated for use: depression in patients who have failed other modes of therapy (tricyclic antidepressants‚ SSRIs‚ SSNRIs‚ or EC therapy). -also used in: panic disorder‚ social anxiety disorder -Action: inhibits the enzyme monoamine oxidase‚ resulting in accumulation of various neurotransmitters (dopamine‚ epinephrine‚ norepinephrine‚ and serotonin) in the body. -Therapeutic effects: improvement in mood for depressed patients
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However‚ there were still inconsistencies in terms of the results that were obtained from this study. The cytochrome B primers all worked as expected‚ with results obtained mirroring the results from the journal. However‚ the mitochondrial cytochrome C oxidase 1 primer did not work at all. There could be several reasons why the primer did not work for this study. Primer inconsistencies usually arise due to incorrect PCR conditions or lack of target sequence in the reaction mix. Usually‚ if the problem
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INTRODUCTION The Gram Negative lab had two parts; the aim of the first part is to determine the concentration of gram negative bacteria in a water sample collected from a creek near Providence Road‚ Strickling. Gram negative bacteria have a cytoplasmic membrane‚ a thin peptidoglycan layer‚ and an additional outer membrane composed of phospholipids and lipopolysaccharides. Because gram negative bacteria have a relatively thin cell wall when compared to gram positive organisms‚ they are consequently
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The word “depressed” and its multiple variations are thrown around casually in every day conversation. Often‚ one will say something like “that movie was so depressing.” Sometimes‚ a person who is feeling blue will describe his mood as depressed‚ but he does not actually have Clinical Depression. Clinical Depression‚ also called Major Depressive Disorder‚ is a serious‚ severe psychological disorder that affects the everyday lives of many individuals. It is actually quite common as “at least 10% of
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