KATTHA INTRODUCTION Kattha & cutch are extracted from wood of Khair tree. Acacia is the botanical name of this tree and it has different varieties like Acacia Sundra‚ Acacia Catechuoides & Acacia Catechu. These species of tree are mainly concentrated in Uttar Pradesh‚ Bihar‚ Rajasthan‚ Gujarat and Himachal Pradesh. The preferred locations are either UP or Bihar. Manufacture of Kattha is an important forest-based traditional industry in India. The Central Forest Research Institute has developed
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Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol Carson Levine November 4th‚ 2013 Abstract Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is exposed to oxygen. When the reaction occurs‚ benzoquinone is produced turning the oxidized substance brown. It was hypothesized that the higher the concentration of catechol oxidase‚ the browner the substance will
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Studying the Rate of Reaction of catechol oxidase and how it affects pH levels Introduction: In this lab‚ we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in
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compounds. In this experiment however‚ the only reaction being tested is the oxidation of catechol (a diphenol) by the enzyme catalyst catechol oxidase or polyphenol oxidase‚ which initiates a chain of reactions and eventually triggers the formation of brown pigments known as catechol-melanins. Catechol‚ also called 1‚2-dihydroxybenzene is a phenolic compound with the chemical formula C6H4(OH)2. The catechol molecule consists of two hydroxyl groups bonded to a central benzene ring‚ hence the molecule
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2) Why is your study significant‚ i.e.‚ how is your objective relevant/applicable to the larger world and why would the information you seek be important/valuable to others? (either in terms of using catechol oxidase as a model to study enzyme function or considering the specific activity of catechol oxidase) 3) State your hypothesis (an educated prediction): Materials and Methods 1) List specific materials used in your experiments (and sources of these materials). Also list any
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infection of microorganisms because it is toxic to them (Danyk‚ H‚ 2013). Quinones are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk‚ H‚ 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract and water. To provide more results of the enzymes productivity patterns the solutions;
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Respectively‚ 1‚ 2‚ 4‚ 8‚ 16‚ and 24 drops of catechol were added to each of the tubes. To equal out the amount of solution in each of the test tubes‚ respectively 23‚ 22‚ 20‚ 16‚ 8 and 0 drops of pH 7 buffer were added. 30 drops of potato juice containing the catechol oxidase enzyme were then added to the solution‚ and then timed for 5 minutes at room temperature. The pH treatment only required 5 test tubes
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Lab: Inhibiting the Action of Catechol Oxidase By: Kimberly G. Introduction: In this lab‚ Mr. Greene ’s sixth period AP Biology class split into groups "to investigate inhibition of enzyme activity by specific chemicals called inhibitors" (1). Group three pondered this lab ’s inhibitor‚ phenylthiourea (PTU). Is it a competitive inhibitor? That is an inhibitor that literally "competes" with the substrate by mimicking it‚ and thus "wins" the position at the active site of the enzyme. The blocked
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insert into cuvettes‚ a micropipette was then used to obtain 0.5ml of catechol and 0.5ml of the catechol oxidase. The pH buffer was made first to avoid any denaturation of the catechol oxidase. Our positive control for this experiment was pH 7 because that is the pH level of most cell membranes in the cytoplasm (Whitson‚ 2016.) Our negative controls varied for each pH buffer‚ but all “blank” cuvettes contained 0.5 ml of catechol oxidase and 4.5ml of the pH buffer. We prepared the negative control
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measuring and recording the different levels of substrate and enzyme we recorded all the data that corresponded to the change after the level of catechol was changed as well as observing the different pH on the same enzyme. Materials List 1. Test tubes 2 .Test tube rack 3. Small Para film squares 4. Distilled water 5. Potato extract 6. Catechol 7. Phenylthiourea 8. Disposable gloves 9. Pipette 5ml 10. Pipette 1ml 11. Bunsen burner 12. Ice 13. Ice chest 14. Thermometer 15. pH solutions
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