procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times) (3 Points) -We had three different test tubes. These test tubes were labeled 1‚ 2‚ and 3. These tubes all had 3.5 mL water‚ 2 mL catechol‚ 0.5 mL extract. Each of these tubes were put into water of three different temperatures for 5 minutes and then were taken to the Spec 20 to check their absorbency. We had a blank tube‚ which measured 1 mL of potato extract and 5 mL of distilled
Premium Boiling Water Distillation
rays‚ and immobilization of toxic ions. There are several types of melanin; eumelanin and pheomelanin (found mostly in animals as L-DOPA)‚ Neuromelanin (found in brain as dopamine‚ norepinephrine‚ and serotonin)‚ Allo Melanin (found in plants‚ as catechol‚ and pyrogallol)‚ Pyomelanin (found in fungi or bacteria as homogentisic acid) Melanin is important to this experiment because‚ the structure of melanin is not fully known. With more knowledge about this chemical‚ and the way it function‚ we would
Premium Psychology Brain Mind
questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate‚ enzyme and product of the enzymatic reaction? 2- What is the purpose of tubes 2B and 2C? Enzyme Specificity Table 2 – Specificity of Cathecol oxidase for different substrates Prediction: _____
Premium Enzyme
Throughout the week in the toxicology lab‚ I was acquainted with distinctive sort of tests and I got the opportunity to find out about the following: 1- hTSH test (Human thyroid stimulating Hormone): - Add calibration and +/- controls‚ before starting the punching process. - Add hTSH assay buffer + stock solution. - Put the plate on a fast quick shaking for 10mins‚ and afterward 4 hours moderate shaking at room T° -Add washing solution. -Add enhancement solution‚ and read the result.
Premium Chemistry Oxygen Water
trigger the monocytes which then become macrophages. The macrophages become activated by the cytokine and begin to attack any tissue in its general vicinity. This could lead to severe tissue damage. (Martz‚ n.d.) Urushiol is made up of a mixture of catechol derivatives‚ the most common found on poison ivy being pentadecylcatechol. Although many people believe that poison ivy is harmless after the plant has died‚ that isn’t the case. Urushiol has been found to stay on a surface for several years. The
Premium Immune system Hypersensitivity
Parkinson’s disease is caused due to the production of insufficient dopamine synthesis in brain….. Degradation of catecholamines The catecholamine is inactivated by oxidative deamination by monoamine Oxidase (MAO) and by o-methylation carried out by catechol-o-methyl-transferase (COMT) as the one-carbon donor. -Two reactions can occur in either direction -the aldehyde products of the MAO reaction are oxidized to the corresponding acids MAO: Inactivates Catecholamine by Oxidative Deamination to
Premium Dopamine Neurotransmitter Epinephrine
these temperatures make the biggest influence. Objects Experiment A: There will be six test tubes are set up‚ which contains one control variable and five different types of substrates: Cyclohexanol‚ (1‚ 4) - Cyclohexandiol‚ (1‚ 2) - Cyclohexanediol‚ Catechol‚ Resourcinol. The darkness of these five test tubes will be the depended variables. Experiment B: There will be eleven test tubes are set up‚ which contains one control variable and ten different types of substrates concentrations : 10%‚ 20%‚ 30%
Premium Enzyme Chemical reaction Metabolism
Part 1 dilutions participated as a trail run to gather around the “full enzyme” that was used for the other parts of the experiment. Part 2 readings were in the time span of 10 minutes and the final results show a spontaneous increase in the first minutes of the absorbance readings. They then showed a steady reading towards the end of timing. The amount of enzyme is decreasing as the dilution occurred. The activity it catalyzes measures the amount of enzyme present in the reaction (Introduction to
Premium Chemistry Chemical reaction Water
PARKINSON’S DISEASE NAME: INSTITUTION: Introduction Parkinson ’s disease is a regarded as a common neurodegenerative condition. The etiology of the disease has not completely been understood‚ but the condition has been associated with a confluence of factors. The first is the loss of a number of neurotransmitters‚ most notably dopamine that produces neurons in the pars compacta of the substantia nigra. It has also been linked to the combined effects of environmental exposures and aging
Premium Parkinson's disease Dopamine Neurology
Dopamine and the related substances norepinephrine (NE) and epinephrine (EPI) comprise a small but important group of neurotransmitters and hormones called catecholamines. * Catecholamine contains two chemical similarities: a core structure of catechol and a nitrogen-containing group called an amine. The catecholamines‚ in turn‚ belong to a wider group of transmitter called either monoamines (transmitters that possess one amine group) or biogenic amines * EPI – adrenergic. NE- noradrenergic
Premium Dopamine