Title: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Aim To design and conduct an experiment to demonstrate the presence of enzyme activity in the preparation provided. To examine the effect of the inhibitors provided. To test whether the other phenolic substrates provided can be oxidized by the enzyme preparation. To test for the presence of peroxidase activity in the enzyme preparation. To test the effect of the inhibitor provided on peroxidase activity
Premium Enzyme Enzyme inhibitor
Effect of Enzyme and Substrate Concentration on Reaction Rate by Zachary A. Poché Biology 155 Laboratory October 15‚ 2014 Lab Partners: Cade White‚ Hannah Ragas‚ Russheka Aremillion ABSTRACT In order to increase the reaction rate‚ substrates attach to the active site of enzymes which decrease the activation energy required to convert substrates to products. We examined the effect of enzyme concentration and substrate concentration on the overall rate of the reaction. To determine
Premium Chemical reaction Chemistry Enzyme
to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant. Our findings illustrate that the rate of enzyme activity is only influenced by substrate concentration at low level of substrate concentration‚ and as substrate concentration increases‚ the
Premium Enzyme Catalysis
The control group of this experiment was room temperature. The constant variables were‚ the amount of distalled water‚ catechol substrate‚ and potato extract in each tube was the same. (2ml) The size of the test tubes were the same also.
Premium Chemical reaction Chemistry Reaction rate
large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped and mixed by inversion. After mixing‚ 1.0mL of catechol was added to each of the five experimental solutions and
Premium Enzyme Chemistry Catalysis
NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
Premium Enzyme
water‚ 6mL of Catechol‚ 1.5mL of Extract(potato)‚ a Spectronic 20 (spectrophotometer)‚ Wax pencil‚ a Test tube rack‚ three Parafilm‚ tissues to clean the Spectronic 20 and the test tubes‚ 3 pipets and a timer. So we won’t confuse ourselves‚ we used the wax pencil to label each test tube as we placed the required amount of substance in each of them. To avoid any slight errors‚ we also used three different pipets for the three required materials‚ which are the distilled water‚ catechol and the extract
Premium Enzyme Laboratory glassware Measurement
Epinephrine Structural Diagram- page 72 [pic] About: - Epinephrine has several uses‚ including increasing ventilation of lungs through relief of bronchodilation‚ increasing cardiac output‚ increasing systolic blood pressure‚ dilating blood vessels involved in crisis response - Clinically‚ epinephrine plays a lifesaving role in countering the effects of anaphylactic shock. It relaxes smooth muscle‚ though at different receptors. Physical Properties: - high boiling points and melting
Premium Heart Epinephrine Asthma
Benzene 1 Benzene Benzene Identifiers [1] CAS number 71-43-2 PubChem 241 ChemSpider 236 UNII J64922108F EC number 200-753-7 KEGG C01407 ChEBI CHEBI:16716 ChEMBL CHEMBL277500 RTECS number CY1400000 Jmol-3D images Image 1 [2] [3] [4] [5] [6] [7] [8] [9] Properties Molecular formula CH Molar mass 78.11 g mol−1 Appearance Colorless liquid Odor aromatic‚ gasoline-like
Premium Benzene
Cyclic voltammograms for 50 moldm-3 catechol were recorded with plain GCE‚ PEDOT-GO‚ PEDOT-rGO and PEDOT-rGO incorporated with LAC at pH 7.0 and scan rate 50 mVs-1 Fig. 4.3.4. Fig 4.3.4A present CVs and the anodic peak current observes for dopamine are bare small redox peak was observed. The modification of PEDOT-GO and PEDOT-rGO were increase in increase in the redox reak compared with bare electrode. The incorporation of LAC on the PEDOT-rGO gives decrease in the oxidation and increase in the
Premium Enzyme Enzyme Electrochemistry