During this experiment‚ the temperature of tubes 5 and 6 showed that the color changed throughout the 20 minute time period. The catechol oxidase has to be at a certain temperature for it to react well. But based on our data when we put it at room temperature the reaction takes longer. If the temperature is too cold then all it does is slow the reaction as well‚ but when it is exposed
Premium Acid Base Chemistry
“CLONING OF HORSE RADISHPEROXIDASE GENE IN E.coli FOR EXTRACELLULAR EXPRESSION” Project report submitted to Jawaharlal Nehru Technological University Hyderabad in partial fulfillment for the award of degree of Bachelor of Technology in Biotechnology Submitted by M.RAVI TEJA (08311A2338) & V.MAYURI (08311A2358) Under the Guidance of DR.CHAND PASHA Department of Microbiology Osmania University [pic] Department Of Biotechnology SREENIDHI INSTITUTE OF SCIENCE AND TECHNOLOGY Yamnampet
Premium Enzyme Molecular biology Oxygen
dopa decarboxylase. Breakdown: Dopamine can be broken down by either reuptake or metabolism. With metabolism dopamine breaks down to 3‚4 DOPAC by monoamine oxidase or dopamine is broken down to 3-MT by catechol 0-methyltransferase. The3‚4 DOPAC than breaks down to homovanillic acid by catechol-o-methyltransferase and 3-MT breaks down to homovanillic acid by monoamine oxidase‚ aldehyde dehydrogenase. (e) Briefly outline one mechanism for the possible genetic basis of alcoholism. GABA is a neurotransmitter
Premium Action potential Neuron Signal transduction
Question 1 1. How is the neurotransmitter acetylcholine inactivated at the parasympathetic terminal receptor site? Answer A. The the enzyme catechol O-methtransferase (COMT) B. By the reuptake process C. By the enzyme cholinesterase D. By the enzyme monoamine oxidase E. By the enzyme nicotinase 1 points Question 2 1. You adminster an inhaled bronchodilator that is known to have adrenergic side effects on the heart. What clnical sign should you watch for in
Premium Asthma
procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times) (3 Points) -We had three different test tubes. These test tubes were labeled 1‚ 2‚ and 3. These tubes all had 3.5 mL water‚ 2 mL catechol‚ 0.5 mL extract. Each of these tubes were put into water of three different temperatures for 5 minutes and then were taken to the Spec 20 to check their absorbency. We had a blank tube‚ which measured 1 mL of potato extract and 5 mL of distilled
Premium Boiling Water Distillation
rays‚ and immobilization of toxic ions. There are several types of melanin; eumelanin and pheomelanin (found mostly in animals as L-DOPA)‚ Neuromelanin (found in brain as dopamine‚ norepinephrine‚ and serotonin)‚ Allo Melanin (found in plants‚ as catechol‚ and pyrogallol)‚ Pyomelanin (found in fungi or bacteria as homogentisic acid) Melanin is important to this experiment because‚ the structure of melanin is not fully known. With more knowledge about this chemical‚ and the way it function‚ we would
Premium Psychology Brain Mind
Throughout the week in the toxicology lab‚ I was acquainted with distinctive sort of tests and I got the opportunity to find out about the following: 1- hTSH test (Human thyroid stimulating Hormone): - Add calibration and +/- controls‚ before starting the punching process. - Add hTSH assay buffer + stock solution. - Put the plate on a fast quick shaking for 10mins‚ and afterward 4 hours moderate shaking at room T° -Add washing solution. -Add enhancement solution‚ and read the result.
Premium Chemistry Oxygen Water
Cyanide (toxin) Name: Kasheba Wright Course: Chemistry in our daily Lives INTRODUCTION Cyanide is a mitochondrial toxin that is among the most rapidly lethal poisons known to man. Used in both ancient and modern times as a method of execution‚ cyanide causes death within minutes to hours of exposure. It is the salts of hydrocyanic acid‚ it is rapidly acting chemical that can exist in various forms
Premium Poison World War II Carbon
trigger the monocytes which then become macrophages. The macrophages become activated by the cytokine and begin to attack any tissue in its general vicinity. This could lead to severe tissue damage. (Martz‚ n.d.) Urushiol is made up of a mixture of catechol derivatives‚ the most common found on poison ivy being pentadecylcatechol. Although many people believe that poison ivy is harmless after the plant has died‚ that isn’t the case. Urushiol has been found to stay on a surface for several years. The
Premium Immune system Hypersensitivity
Parkinson’s disease is caused due to the production of insufficient dopamine synthesis in brain….. Degradation of catecholamines The catecholamine is inactivated by oxidative deamination by monoamine Oxidase (MAO) and by o-methylation carried out by catechol-o-methyl-transferase (COMT) as the one-carbon donor. -Two reactions can occur in either direction -the aldehyde products of the MAO reaction are oxidized to the corresponding acids MAO: Inactivates Catecholamine by Oxidative Deamination to
Premium Dopamine Neurotransmitter Epinephrine